Novo, MercedesAl-Soufi, Wajih2025-01-272025-01-272022-10-31Novo M and Al-Soufi W (2022), Supramolecular association studied by Fluorescence correlation spectroscopy. Front. Chem. 10:1042658. doi: 10.3389/fchem.2022.10426582296-2646https://hdl.handle.net/10347/39050A comprehensive description of a supramolecular system involves a full understanding of its thermodynamic and dynamic properties, as well as detailed knowledge of its structure. Fluorescence Correlation Spectroscopy (FCS) constitutes a powerful technique to acquire this information. Fluorescence correlation curves show a characteristic diffusion term that is related to the binding equilibrium constant or other thermodynamic properties of the supramolecular system. The association and dissociation rate constants of the binding process can be determined in FCS when the relaxation time of the binding is faster than the observation time—a regime called fast-exchange dynamics - in opposition to the slow-exchange regime. In all cases, structural information can be inferred from the diffusional properties of the supramolecular complexes. A short overview of the use of FCS for the study of supramolecular systems is given with examples which belong to the fast and slow regime.eng© 2022 Novo and Al-Soufi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY).http://creativecommons.org/licenses/by/4.0/Fluorescence Correlation SpectroscopySupramolecular associationSlow exchangeFast exchangeBinding constantAssociation dynamicsjournal article10.3389/fchem.2022.1042658open access