Fuentes Alonso, ElsaVázquez Nión, DanielPrieto Lamas, Beatriz2022-11-232022-11-232022Building and Environment 223 (2022) 109451http://hdl.handle.net/10347/29459Study of the biodeterioration of building materials is often hampered by the inaccessibility of the colonization and impossibility of sampling in protected buildings considered part of the cultural heritage. There is therefore a recognised need to develop a laboratory mesocosm that realistically represents nature and enables the study and analysis of both the substrate and the organisms involved. Although many studies have investigated the formation of biofilms on various building materials, there is a lack of homogeneity and consensus in the methods and protocols used, which hampers comparison of the results obtained. This review aims to identify the different methods reported in the scientific literature, to organise these according to different factors (water access and types of organism and substrata used) and thus provide the research community with a guide for selecting the most appropriate methods according to the different objectiveseng© 2022 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by- nc-nd/4.0/)Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/BuildingsBiodeteriorationMethodologiesSubaerial biofilms formationjournal article10.1016/j.buildenv.2022.1094510360-1323open access