Brea López, DavidAgulla Freire, JesúsStaes, AnGevaert, KrisCampos Pérez, FranciscoSobrino Moreiras, TomásBlanco González, MiguelDávalos, AntoniCastillo Sánchez, José AntonioRamos Cabrer, Pedro2020-04-242020-04-242015Brea, D. et al. Study of Protein Expresion in Peri-Infarct Tissue after Cerebral Ischemia. Sci. Rep. 5, 12030; doi: 10.1038/srep12030 (2015).http://hdl.handle.net/10347/21731In this work, we report our study of protein expression in rat peri-infarct tissue, 48h after the induction of permanent focal cerebral ischemia. Two proteomic approaches, gel electrophoresis with mass spectrometry and combined fractional diagonal chromatography (COFRADIC), were performed using tissue samples from the periphery of the induced cerebral ischemic lesions, using tissue from the contra-lateral hemisphere as a control. Several protein spots (3408) were identified by gel electrophoresis, and 11 showed significant differences in expression between peri-infarct and contralateral tissues (at least 3-fold, p<0.05). Using COFRADIC, 5412 proteins were identified, with 72 showing a difference in expression. Apart from blood-related proteins (such as serum albumin), both techniques showed that the 70kDa family of heat shock proteins were highly expressed in the periinfarct tissue. Further studies by 1D and 2D western blotting and immunohistochemistry revealed that only one member of this family (the inducible form, HSP72 or HSP70i) is specifically expressed by the peri-infarct tissue, while the majority of this family (the constitutive form, HSC70 or HSP70c) is expressed in the whole brain. Our data support that HSP72 is a suitable biomarker of peri-infarct tissue in the ischemic brain.engCopyright © 2015, The Author(s). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/http://creativecommons.org/licenses/by/4.0/journal article10.1038/srep120302045-2322open access