Liñares, AntonioRubinos da Silva, Ana MaríaPuñal, AnaMuñoz Guzón, Fernando MaríaBlanco Carrión, Juan2022-09-282022-09-282022Liñares, A., Rubinos, A., Puñal, A.,Muñoz, F., & Blanco, J. (2022). Regeneration of keratinizedtissue around teeth and implants following coronalrepositioning of alveolar mucosa with and without aconnective tissue graft: An experimental study in dogs.Journalof Clinical Periodontology,1–12.https://doi.org/10.1111/jcpe.1367312http://hdl.handle.net/10347/29279Aim: To compare clinical and histological keratinized tissue formation around teeth and implants following coronal repositioning of alveolar mucosa with or without a connective tissue graft (CTG).Materials and Methods: In nine beagle dogs, the third and fourth premolars (P3 and P4) were extracted from one side of the maxilla. Three months after the tooth extraction, a full-thickness buccal flap was raised and two implants were placed in those healed areas. On the contra-lateral side, a buccal flap was also raised at the P3 and P4 areas. Before suturing, the dogs were randomly assigned to three study groups (control, non-keratinized tissue [NKT], and non-keratinized tissue CTG [NKT-CTG]). In the control group, the buccal flaps were re-positioned around the teeth (P3 and P4) on one side, and implants on the other side, presenting an adequate band of keratinized tissue (KT). For the NKT and NKT-CTG groups, this buccal KT was then excised. In the NKT group, the buccal flap without KT (alveolar mucosa) was re-positioned around the teeth and implants. In the NKT-CTG group, a CTG taken from the excised KT was sutured to the buccal alveolar mucosa and then both were re-positioned around the teeth and implants. The clinical height of the KT was measured at baseline and at 1, 2, and 3 months of healing. The animals were sacrificed at 3 months, at which point the KT height was measured histologically. Results: The control group presented normal healing with a band of KT surrounding the teeth and implants. In the NKT and NKT-CTG groups, a new KT band approximately 2 mm in height (measured clinically and histologically) spontaneously formed around all teeth, regardless of whether a CTG had been placed. In the NKT implant group, no new KT was observed (clinically or histologically). Around the implants in the NKT-CTG group, a small amount of KT was formed in just two of the six implants.Conclusions: After surgical excision of KT, spontaneous KT is formed around teeth but not around implants, regardless of the placement of a CTGeng© 2022 The Authors. Journal of Clinical Periodontology published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.Atribución-NoComercial 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc/4.0/Connective tissue graftImplantsKeratinized tissueSoft tissue healingTeethjournal article10.1111/jcpe.136731600-051Xopen access