González Jartín, Jesús MaríaRodríguez Cañás, InésAlfonso Rancaño, María AmparoSáinz Oses, María JesúsRodríguez Vieytes, MercedesGomes, AnaRamos, IsabelBotana López, Luis Miguel2025-12-182025-12-182021-09-15Food Chemistry Volume 356, 15 September 2021, 1296470308-8146https://hdl.handle.net/10347/44562A simple method for the quantification of 40 mycotoxins in milk was developed. This method is based on a QuEChERS extraction followed by the ultra-high liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) detection, and allows the simultaneous analysis of regulated, emerging, and modified mycotoxins. A sample treatment procedure was optimized to include a concentration step for the analysis of some compounds such as aflatoxin M1. The method was in-house validated in terms of limits of detection (LODs), limits of quantification (LOQs), linearity, recoveries, and precision. LOQs lower than 10 ng/mL were obtained, and recoveries ranged from 61% to 120% with a precision, expressed as the relative standard deviation, lower than 15%. Therefore, acceptable performance characteristics were obtained fulfilling European regulations. The method was successfully applied for the quantification of mycotoxins in raw milk. It can be highlighted high occurrence of beauvericin and enniatins were found in low amounts.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/MycotoxinMilkQuEChERSUHPLC-MS/MSjournal article10.1016/j.foodchem.2021.129647open access