Rodríguez Filgueiras, InésAlfonso Rancaño, María AmparoAntelo Queijo, ÁlvaroÁlvarez, MercedesBotana López, Luis Miguel2017-11-102017-11-102016-06Rodríguez, I., Alfonso, A., Antelo, A., Alvarez, M., & Botana, L. M. (2016). Evaluation of the impact of mild steaming and heat treatment on the concentration of okadaic acid, dinophysistoxin-2 and dinophysistoxin-3 in mussels. Toxins, 8(6), 175.2072-6651http://hdl.handle.net/10347/16131This study explores the effect of laboratory and industrial steaming on mussels with toxin concentrations above and below the legal limit. We used mild conditions for steaming, 100 ˝C for 5 min in industrial processing, and up to 20 min in small-scale laboratory steaming. Also, we studied the effect of heat on the toxin concentration of mussels obtained from two different locations and the effect of heat on the levels of dinophysistoxins 3 (DTX3) in both the mussel matrix and in pure form (7-O-palmitoyl okadaic ester and 7-O-palmytoleyl okadaic ester). The results show that the loss of water due to steaming was very small with a maximum of 9.5%, that the toxin content remained unchanged with no concentration effect or increase in toxicity, and that dinophysistoxins 3 was hydrolyzed or degraded to a certain extent under heat treatment. The use of liquid-certified matrix showed a 55% decrease of dinophysistoxins 3 after 10 min steaming, and a 50% reduction in total toxicity after treatment with an autoclave (121 ˝C for 20 min)engCopyright by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/SteamingOkadaic acidMass spectrometryDinophysistoxinMaterias::Investigación::32 Ciencias médicas::3209 Farmacologíajournal article10.3390/toxins8060175open access