A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves

Rodríguez Villar, JéssicaMosquera Mosquera, JesúsGarcía Fandiño, RebecaVázquez Sentís, Marco EugenioMascareñas Cid, José Luis2017-10-212017-10-212016-02-05J. Rodríguez, J. Mosquera, R. García-Fandiño, M. E. Vázquez and J. L. Mascareñas, Chem. Sci., 2016, 7, 3298–33032041-6539http://hdl.handle.net/10347/16016We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and selectivity to a long composite sequence of 13 base pairs (TCAT-AATT-GAGAG)eng© The author(s) 2016. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported LicenceA designed DNA binding motif that recognizes extended sites and spans two adjacent major groovesjournal article10.1039/c6sc00045bopen access