Santás Miguel, VanesaRodríguez González, LauraNúñez Delgado, AvelinoDíaz Raviña, MontserratArias Estévez, ManuelFernández Calviño, David2020-12-162020-12-162020Santás-Miguel, V.; Rodríguez-González, L.; Núñez-Delgado, A.; Díaz-Raviña, M.; Arias-Estévez, M.; Fernández-Calviño, D. The Toxicity Exerted by the Antibiotic Sulfadiazine on the Growth of Soil Bacterial Communities May Increase over Time. Int. J. Environ. Res. Public Health 2020, 17, 8773http://hdl.handle.net/10347/24029The toxicity exerted by the antibiotic sulfadiazine on the growth of soil bacterial communities was studied in two agricultural soils for a period of 100 days. In the short-term (2 days of incubation), the effect of sulfadiazine on bacterial growth was low (no inhibition or inhibition <32% for a dose of 2000 mg·kg−1). However, sulfadiazine toxicity increased with time, achieving values of 40% inhibition, affecting bacterial growth in both soils after 100 days of incubation. These results, which were here observed for the first time for any antibiotic in soil samples, suggest that long-term experiments would be required for performing an adequate antibiotics risk assessment, as short-term experiments may underestimate toxicity effectseng© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Leucine incorporationRisk assessmentSulfonamideVeterinaryMicrobial communityjournal article10.3390/ijerph172387731660-4601open access