Souto Pereira, SandraOlveira Hermida, José G.Bandín Matos, Isabel2026-02-202026-02-202015-07-04Souto S, Olveira JG, Bandín I. Influence of temperature on Betanodavirus infection in Senegalese sole (Solea senegalensis). Vet Microbiol. 2015 Sep 30;179(3-4):162-7. doi: 10.1016/j.vetmic.2015.07.004. Epub 2015 Jul 8. PMID: 26187680https://hdl.handle.net/10347/46011In this study Senegalese sole juveniles were experimentally infected with a reassortant Betanodavirus strain at three different temperatures: 22 °C, 18 °C and 16 °C by bath challenge and cohabitation. The results obtained showed that virus virulence decreased by reducing the water temperature. At 22 °C mortalities reached 100%, at 18 °C they ranged from 75 to 80% and at 16 °C only 8% of the fish died. In addition, horizontal transmission was demonstrated regardless of the rearing temperature. At 16 °C active viral replication was detected up to 66 days post-infection, but no signs of the disease were observed and only a very low level of mortality was recorded. The increase in water temperature from 16 to 22 °C caused a quick rise in the viral load and a subsequent outbreak of mortalities. These findings demonstrate that this reassortant Betanodavirus strain can cause a persistent infection in Senegalese sole at low temperatures (16 °C) for long periods of time, and when temperature increases the virus is able to trigger an acute infection and provoke high mortalitiesenghttp://creativecommons.org/licenses/by-nc-nd/4.0/BetanodavirusHorizontal transmissionReassortantSenegalese soleViral loadWater temperature24 Ciencias de la vidajournal article10.1016/j.vetmic.2015.07.0041873-2542open access