Fernández Sánchez, Francisco IsmaelBarrio López, MónicaQuintela Arias, Luis ÁngelBecerra González, Juan JoséPeña Martínez, Ana IsabelMartínez Bello, DanielGarcía Herradón, Pedro JoséPérez Marín, Carlos C.2020-05-252020-05-252014Fernández Sánchez, F.I., Barrio López, M., Quintela Arias, L.A., Becerra González, J.J., Peña Martínez, A.I., Martínez Bello, D. et al.(2014), Use of endometrial cytology and metabolic profiles for selection of embryo donor cows. Span. J. Agric. Res. vol. 12 (3), 664-6711695-971Xhttp://hdl.handle.net/10347/22555The aim of this study was to evaluate the use of endometrial cytology and metabolic profiles for selection of donor cows in embryo transfer programmes. For this purpose, 69 clinically healthy Holstein cows were enrolled in the study. At the start of the superovulation procedure (Day 0), blood and endometrial samples were obtained to determine metabolic and uterine status, respectively. The cows were then subjected to porcine follicle stimulating hormone (pFSH) superovulation treatment, and embryos were recovered after 7 days. The mean number of embryos obtained per flush was 9.89 ± 8.21 (4.63 ± 5.34 viable embryos, 0.82 ± 2.01 degenerated embryos and 4.57 ± 6.44 unfertilized ova). The following statistically significant variables were entered in a regression model: beta-hydroxybutyrate, serum cholesterol, body condition, number of calvings and percentage of neutrophils. In almost all cases, the model explained some percentage of the variance: total number of embryos, 4.8% (p < 0.05); number of degenerate embryos, 4.2% (p = 0.051); and number of unfertilized ova, 14.2% (p < 0.01). Statistical models for the percentage of viable embryos and unfertilized ova accounted for 24.0% and 29.4% of the variance, respectively, and both were statistically significant (p < 0.01). The model for the percentage of degenerated embryos was statistically significant (p < 0.05) and explained 4.4% of the variance. In conclusion, we have demonstrated that positive energy balance and healthy uterus can improve ovarian response and the proportion of viable embryos in cows. Efficient tools for monitoring the metabolic and uterine status should therefore be used in bovine embryo transfer programmeseng© INIA, 2014. This work is distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) Licensehttps://creativecommons.org/licenses/by/4.0/Dairy cattleEmbryo transferSerum chemistry parametersEndometritisNeutrophilsjournal article10.5424/sjar/2014123-49482171-9292open access