RT Journal Article
T1 Use of Quorum Sensing Inhibition Strategies to Control Microfouling
A1 Muras Mora, Andrea
A1 Parga Martínez, Ana
A1 Mayer Mayer, Celia
A1 Otero Casal, Ana María
K1 Microfouling
K1 Biofilm
K1 Marine bacteria
K1 Quorum sensing
K1 Quorum quenching
K1 Lactonase
AB Interfering with the quorum sensing bacterial communication systems has been proposed as a promising strategy to control bacterial biofilm formation, a key process in biofouling development. Appropriate in vitro biofilm-forming bacteria models are needed to establish screening methods for innovative anti-biofilm and anti-microfouling compounds. Four marine strains, two Pseudoalteromonas spp. and two Vibrio spp., were selected and studied with regard to their biofilm-forming capacity and sensitivity to quorum sensing (QS) inhibitors. Biofilm experiments were performed using two biofilm cultivation and quantification methods: the xCELLigence® system, which allows online monitoring of biofilm formation, and the active attachment model, which allows refreshment of the culture medium to obtain a strong biofilm that can be quantified with standard staining methods. Although all selected strains produced acyl-homoserine-lactone (AHL) QS signals, only the P. flavipulchra biofilm, measured with both quantification systems, was significantly reduced with the addition of the AHL-lactonase Aii20J without a significant effect on planktonic growth. Two-species biofilms containing P. flavipulchra were also affected by the addition of Aii20J, indicating an influence on the target bacterial strain as well as an indirect effect on the co-cultured bacterium. The use of xCELLigence® is proposed as a time-saving method to quantify biofilm formation and search for eco-friendly anti-microfouling compounds based on quorum sensing inhibition (QSI) strategies. The results obtained from these two in vitro biofilm formation methods revealed important differences in the response of biosensor bacteria to culture medium and conditions, indicating that several strains should be used simultaneously for screening purposes and the cultivation conditions should be carefully optimized for each specific purpose
PB MDPI
YR 2021
FD 2021
LK http://hdl.handle.net/10347/24451
UL http://hdl.handle.net/10347/24451
LA eng
NO Mar. Drugs 2021, 19(2), 74; https://doi.org/10.3390/md19020074
NO This work was supported by the European project BYEFOULING “Low-toxic cost-efficient environment-friendly antifouling materials” (FP7-OCEAN-2013 612717) and the project PID2019-104439RB-C21/AEI/10.13039/501100011033 of the Agencia Estatal de Investigación (AEI, Spain), and co-funded by the European Regional Development Fund of the European Union: A Way to Making Europe (FEDER). A.M. and A.P. were supported by predoctoral fellowships from the Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia (ED481A-2015/311 and ED481A-2019/194). C.M. was supported by a postdoctoral fellowship from Xunta de Galicia (IN606B-2019/010)
DS Minerva
RD 28 abr 2026