RT Journal Article
T1 Microfluidic devices manufacturing with a stereolithographic printer for biological applications
A1 Carnero Groba, Bastián
A1 Bao Varela, María del Carmen
A1 Gómez Varela, Ana Isabel
A1 Álvarez Castro, Ezequiel
A1 Flores Arias, María Teresa
K1 Stereolithography
K1 Low force stereolithography
K1 Microfluidics
K1 Organ-on-a-chip
K1 3D printing
K1 Microchannel
K1 Pillar
K1 Soft lithography
K1 PDMS
K1 Biocompatibility
K1 HUVEC
AB Stereolithographic printers have revolutionized many manufacturing processes with their capacity to easily produce highly detailed structures. In the field of microfluidics, this technique avoids the use of complex steps and equipment of the conventional technologies. The potential of low force stereolithography technology is analysed for the first time using a Form 3B printer and seven printing resins through the fabrication of microchannels and pillars. Manufacturing performance of internal and superficial channels and pillars is studied for the seven printing resins in different configurations. A complete characterization of printed structures is carried out by optical, confocal and SEM microscopy, and EDX analysis. Internal channels with unobstructed lumen are obtained for diameters and angles greater than 500 μm and 60°, respectively. Outward and inward superficial channels in the range of hundreds of microns can be fabricated with an accurate profile, printing them with a perpendicular orientation respect to the base, allowing a proper uncured resin evacuation. Outward channels are replicated by soft lithography using polydimethylsiloxane. Clear, Model and Tough resins show a good behaviour to be used as master, but Amber and Dental resins present a poor topology transference from the master to the replica. According to the needs of devices used for biological and biomedical research, transparency as well as superficial biocompatibility of some resins is evaluated. Human umbilical vein endothelial cells (HUVEC) adhesion is confirmed on Amber, Dental and Clear resins, but these cells were only able to grow and progress as a cell culture over the Amber resin. Therefore, Amber showed an adequate biocompatibility, in terms of cell adhesion and growth for HUVEC
PB Elsevier
YR 2021
FD 2021
LK http://hdl.handle.net/10347/27676
UL http://hdl.handle.net/10347/27676
LA eng
NO Materials Science and Engineering C 129 (2021) 112388. https://doi.org/10.1016/j.msec.2021.112388
NO Authors gratefully thank contracts AEI RTI2018-097063-B-100, AEI/FEDER, UE; ED431B 2020/29; ED431E 2018/08 and ED481D-2021-019, Consellería de Educación Xunta de Galicia/FEDER e Estructuración Xunta de Galicia, IN607A2019-02 and Sociedad española de cardiología y Fundación español del corazón, SEC/FEC-INV-BAS 20/013
DS Minerva
RD 22 abr 2026