RT Journal Article
T1 Antimicrobial cyclodextrin-assisted electrospun fibers loaded with carvacrol, citronellol and cinnamic acid for wound healing
A1 Gonzalez-Prada, Iago
A1 Borges, Anabela
A1 Santos-Torres, Beatriz
A1 Magariños Ferro, Beatriz
A1 Simões, Manuel
A1 Concheiro Nine, Ángel Joaquín
A1 Álvarez Lorenzo, Carmen
K1 Electrospinning
K1 Cyclodextrin
K1 Essential oil components
K1 Antimicrobial
K1 Antioxidant
K1 In ovo CAM test
AB This work aimed to explore an alternative to the use of antibiotics for prevention and treatment of wounds infection caused by two common bacterial pathogens Staphylococcus aureus and Pseudomonas aeruginosa. For this purpose, three different essential oil components (EOCs), namely carvacrol, citronellol and cinnamic acid, were loaded into electrospun fibers of poly-ε-caprolactone (PCL) aided by alpha-cyclodextrin (αCD) and hydroxypropyl-β-cyclodextrin (HPβCD). Electrospun-fibers prepared with each EOC and their mixtures were screened for antimicrobial capability and characterized regarding morphological, mechanical, thermal, surface polarity, antibiofilm and antioxidant properties. αCD formed poly(pseudo)rotaxanes with PCL and weakly interacted with EOCs, while HPβCD facilitated EOC encapsulation and formation of homogeneous fibers (500–1000 nm diameter) without beads. PCL/HPβCD fibers with high concentration of EOCs (mainly carvacrol and cinnamic acid) showed strong antibiofilm (>3 log CFU reduction) and antioxidant activity (10–50% DPPH scavenging effects). Different performances were recorded for the EOCs and their mixtures; cinnamic acid migrated to fiber surface and was released faster. Fibers biocompatibility was verified using hemolysis tests and in ovo tissue integration and angiogenesis assays. Overall, HPβCD facilitates complete release of EOCs from the fibers to the aqueous medium, being an environment-friendly and cost-effective strategy for the treatment of infected wounds.
PB Elsevier
SN 0141-8130
YR 2024
FD 2024-08-09
LK https://hdl.handle.net/10347/39751
UL https://hdl.handle.net/10347/39751
LA eng
NO References  Gonzalez-Prada, I., Borges, A., Santos-Torres, B., Magariños, B., Simões, M., Concheiro, A., & Alvarez-Lorenzo, C. (2024). Antimicrobial cyclodextrin-assisted electrospun fibers loaded with carvacrol, citronellol and cinnamic acid for wound healing. International Journal of Biological Macromolecules, 27710.1016/j.ijbiomac.2024.134154
NO The work was supported by MCIN [PID 2020-113881RB-I00/AEI/10.13039/501100011033], Spain, FEDER and Xunta de Galicia [ED431C 2024/09]. The work was also partially granted by the Spanish Ministry of Science and Innovation with funds from the European Union NextGenerationEU, from the Recovery, Transformation and Resilience Plan (PRTR-C17.I1) and the Autonomous Community of Galicia within the framework of the Biotechnology Plan Applied to Health. This work was also partially supported by the European Commission (Horizon-Widera 2023-Access-02/Horizon-CSA) [InnovAntiBiofilm, ref. 101157363], and LEPABE, UIDB/00511/2020 [DOI: 10.54499/UIDB/00511/2020] and UIDP/00511/2020 [DOI: 10.54499/UIDP/00511/2020]; ALiCE, LA/P/0045/2020 [DOI: 10.54499/LA/P/0045/2020]; and the FCT/MCTES (PIDDAC; Lisbon, Portugal).
DS Minerva
RD 23 abr 2026