RT Journal Article
T1 A bio-inspired hypoxia sensor using HIF1a-oxygen-dependent degradation domain
A1 Iglesias, Pablo
A1 Penas Tallón, Cristina
A1 Barral Cagiao, Laura
A1 Pazos Chantrero, Elena
A1 Costoya Puente, José Antonio
K1 Chemical biology
K1 Cancer
AB Functional imaging has become an important tool in oncology because it not only provides information about the size and localization of the tumour, but also about the pathophysiological features of the tumoural cells. One of the characteristic features of some tumour types is that their fast growth leads to defcient intratumoral vascularization, which results in low oxygen availability. To overcome this lack of oxygen, tumoural cells activate the neoangiogenic program by upregulating the transcription factor HIF-1α. Herein we report a non-invasive in vitro detection method of hypoxia using designed fuorescent peptide probes based on the oxygen-dependent degradation domain of HIF-1α. The fuorescent probe retains the oxygen-sensing capability of HIF-1α, so that it is stabilized under hypoxia and readily degraded by the proteasome under normoxia, thus providing direct information of the cellular oxygen availability
PB Nature Publishing Group
YR 2019
FD 2019
LK http://hdl.handle.net/10347/21556
UL http://hdl.handle.net/10347/21556
LA eng
NO Iglesias, P., Penas, C., Barral-Cagiao, L. et al. (2019). A Bio-inspired Hypoxia Sensor using HIF1a-Oxygen-Dependent Degradation Domain. Sci Rep 9, 7117. doi:10.1038/s41598-019-43618-4
NO This work has received financial support from the Spanish MICINN (SAF2009-08629; J.A.C.) and ISCIII, MINECO (PI15/01129), AEI/FEDER, UE (CTQ2017-89166-R; E.P.), the Consellería de Cultura, Educación e Ordenación Universitaria (GPC2014/030, INCITE08PXIB208091PR; J.A.C.), the Xunta de Galicia (Centro Singular de Investigación de Galicia accreditations 2016‐2019, ED431G/05 and ED431G/09) and the European Regional Development Fund (ERDF). C.P. thanks the Spanish MICINN for her PhD fellowship. E.P. thanks the UDC-Inditex InTalent Programme for her research contract and funding
DS Minerva
RD 23 abr 2026