RT Journal Article
T1 3D Printed Tacrolimus Rectal Formulations Ameliorate Colitis in an Experimental Animal Model of Inflammatory Bowel Disease
A1 Seoane Viaño, Iria
A1 Gómez Lado, Noemí
A1 Lázare Iglesias, Héctor
A1 García Otero, Xurxo
A1 Antúnez López, José Ramón
A1 Ruibal Morell, Álvaro
A1 Varela Correa, Juan Jesús
A1 Aguiar Fernández, Pablo
A1 Basit, Abdul W.
A1 Otero Espinar, Francisco Javier
A1 González Barcia, Miguel
A1 Goyanes Goyanes, Álvaro
A1 Luzardo Álvarez, Asteria María
A1 Fernández Ferreiro, Anxo
K1 Three-dimensional printing
K1 PET/CT imaging
K1 Rectal drug delivery
K1 Ulcerative colitis
K1 TNBS rat model
K1 M3dimaker
K1 3D printed drug products
K1 Personalized medicines and pharmaceuticals
AB The aim of this study was to fabricate novel self-supporting tacrolimus suppositories using semisolid extrusion 3-dimensional printing (3DP) and to investigate their efficacy in an experimental model of inflammatory bowel disease. Blends of Gelucire 44/14 and coconut oil were employed as lipid excipients to obtain suppository formulations with self-emulsifying properties, which were then tested in a TNBS (2,4,6-trinitrobenzenesulfonic acid) induced rat colitis model. Disease activity was monitored using PET/CT medical imaging; maximum standardized uptake values (SUVmax), a measure of tissue radiotracer accumulation rate, together with body weight changes and histological assessments, were used as inflammatory indices to monitor treatment efficacy. Following tacrolimus treatment, a significant reduction in SUVmax was observed on days 7 and 10 in the rat colon sections compared to non-treated animals. Histological analysis using Nancy index confirmed disease remission. Moreover, statistical analysis showed a positive correlation (R2 = 71.48%) between SUVmax values and weight changes over time. Overall, this study demonstrates the effectiveness of 3D printed tacrolimus suppositories to ameliorate colitis and highlights the utility of non-invasive PET/CT imaging to evaluate new therapies in the preclinical area
PB MDPI
YR 2020
FD 2020
LK http://hdl.handle.net/10347/24017
UL http://hdl.handle.net/10347/24017
LA eng
NO Seoane-Viaño, I.; Gómez-Lado, N.; Lázare-Iglesias, H.; García-Otero, X.; Antúnez-López, J.R.; Ruibal, Á.; Varela-Correa, J.J.; Aguiar, P.; Basit, A.W.; Otero-Espinar, F.J.; González-Barcia, M.; Goyanes, A.; Luzardo-Álvarez, A.; Fernández-Ferreiro, A. 3D Printed Tacrolimus Rectal Formulations Ameliorate Colitis in an Experimental Animal Model of Inflammatory Bowel Disease. Biomedicines 2020, 8, 563
NO This research was funded by Xunta de Galicia grant number GRC2013/015 and GPC2017/015. A.F.-F. acknowledges the support of Instituto de Salud Carlos III (Juan Rodés research grant JR18/00014). P.A. acknowledges the support of RYC-2015/17430 (Ramón y Cajal). X.G.-O. acknowledges the financial support of the IDIS (Health Research Institute of Santiago de Compostela)
DS Minerva
RD 30 abr 2026