RT Dissertation/Thesis
T1 Orthogonal Metal Catalysis in Biological Systems
A1 Gutiérrez González, Alejandro
K1 Química Orgánica
K1 Bioortogonal
K1 Organometálica
K1 Complejos
K1 Química Biológica
AB Over the past two decades, the development of bioorthogonal chemistry has revolutionized the chemical tools available for cell research. In this thesis, we have developed novel ruthenium complexes that enable ruthenium-catalyzed azide-thioalkyne cycloadditions to occur under highly efficient micromolar conditions, even in the presence of cells. Moreover, we have successfully translated rutheniumcatalyzed alkene-alkyne coupling into aqueous and bioorthogonal conditions, enabling the modification of peptides to occur efficiently under micromolar conditions. Furthermore, we have engineered a new ruthenium isomerization reaction that can take place inside living mammalian cells, which can be used to effectively deplete the natural cell levels of GSH. Finally, we have demonstrated that discrete organometallic catalysts can be used in combination with natural enzymes to create a primitive artificial metabolic network, even inside living cells.
YR 2023
FD 2023
LK http://hdl.handle.net/10347/30433
UL http://hdl.handle.net/10347/30433
LA eng
DS Minerva
RD 24 abr 2026