RT Journal Article
T1 Circulating Tumor Cells Characterization Revealed TIMP1 as a Potential Therapeutic Target in Ovarian Cancer
A1 Abreu Rodríguez, Manuel
A1 Cabezas Sáinz, Pablo
A1 Alonso Alconada, Lorena
A1 Ferreirós, Alba
A1 Mondelo Macía, Patricia
A1 Lago Lestón, Ramón
A1 Abalo, Alicia
A1 Díaz, Eva
A1 Palacios Zambrano, Sara
A1 Rojo Sebastián, Alejandro
A1 López López, Rafael
A1 Sánchez Piñón, Laura
A1 Moreno Bueno, Gema
A1 Muinelo Romay, Laura
K1 Ovarian cancer
K1 Circulating tumor cells (CTCs)
K1 Cell plasticity
K1 TIMP1
AB Background: Recent studies showed a relevant role of hematogenous spread in ovarian cancer and the interest of circulating tumor cells (CTCs) monitoring as a prognosis marker. The aim of the present study was the characterization of CTCs from ovarian cancer patients, paying special attention to cell plasticity characteristics to better understand the biology of these cells. Methods: CTCs isolation was carried out in 38 patients with advanced high-grade serous ovarian cancer using in parallel CellSearch and an alternative EpCAM-based immunoisolation followed by RT-qPCR analysis to characterize these cells. Results: Epithelial CTCs were found in 21% of patients, being their presence higher in patients with extraperitoneal metastasis. Importantly, this population was characterized by the expression of epithelial markers as MUC1 and CK19, but also by genes associated with mesenchymal and more malignant features as TIMP1, CXCR4 and the stem markers CD24 and CD44. In addition, we evidenced the relevance of TIMP1 expression to promote tumor proliferation, suggesting its interest as a therapeutic target. Conclusions: Overall, we evidenced the utility of the molecular characterization of EpCAM+ CTCs from advanced ovarian cancer patients to identify biomarkers with potential applicability for disseminated disease detection and as therapeutic targets such as TIMP1
PB MDPI
YR 2020
FD 2020
LK http://hdl.handle.net/10347/23866
UL http://hdl.handle.net/10347/23866
LA eng
NO Abreu, M.; Cabezas-Sainz, P.; Alonso-Alconada, L.; Ferreirós, A.; Mondelo-Macía, P.; Lago-Lestón, R.M.; Abalo, A.; Díaz, E.; Palacios-Zambrano, S.; Rojo-Sebastian, A.; López-López, R.; Sánchez, L.; Moreno-Bueno, G.; Muinelo-Romay, L. Circulating Tumor Cells Characterization Revealed TIMP1 as a Potential Therapeutic Target in Ovarian Cancer. Cells 2020, 9, 1218
NO Part of this research was supported by CIBERONC funds (CB16/12/00328)
DS Minerva
RD 24 abr 2026