RT Journal Article
T1 Hybrid gold nanorod-based nanoplatform with chemo and photothermal activities for bimodal cancer therapy
A1 Arellano, Lilia G.
A1 Villar Álvarez, Eva
A1 Varela, Alejandro
A1 Figueroa, Valeria
A1 Fernández Vega, Javier
A1 Cambón Freire, Adriana
A1 Prieto Estévez, Gerardo
A1 Barbosa Fernández, Silvia
A1 Taboada Antelo, Pablo
K1 Gold nanorods
K1 Layer assembly
K1 Photothermal therapy
K1 Multimodal therapy
K1 Cell death pathway
AB Metal nanoparticles (NPs), particularly gold nanorods (AuNRs), appear as excellent platforms not only to transport and deliver bioactive cargoes but also to provide additional therapeutic responses for diseased cells and tissues and/or to complement the action of the carried molecules. In this manner, here, we optimized a previous developed metal-based nanoplatform composed of an AuNR core surrounded by a polymeric shell constructed by means of the layer-by-layer approach, and in which very large amounts of the antineoplasic drug doxorubicin (DOXO) in a single loading step and targeting capability thanks to an outer hyaluronic acid layer were incorporated by means of an optimized fabrication process (PSS/DOXO/PLL/HA-coated AuNRs). The platform retained its nanometer size with a negative surface charge and was colloidally stable in a range of physiological conditions, in which only in some of them some particle clustering was noted with no precipitation. In addition, the dual stimuli-responsiveness of the designed nanoplatform to both endogenous proteases and external applied light stimuli allows to perfectly manipulate the chemodrug release rates and profiles to achieve suitable pharmacodynamics. It was observed that the inherent active targeting abilities of the nanoplatfom allow the achievement of specific cell toxicity in tumoral cervical HeLa cells, whilst healthy ones such as 3T3-Balb fibroblast remain safe and alive in agreement with the detected levels of internalization in each cell line. In addition, the bimodal action of simultaneous chemo- and photothermal bioactivity provided by the platform largely enhances the therapeutic outcomes. Finally, it was observed that our PSS/DOXO/PLL/HA-coated AuNRs induced cell mortality mainly through apoptosis in HeLa cells even in the presence of NIR light irradiation, which agrees with the idea of the chemo-activity of DOXO predominating over the photothermal effect to induce cell death, favoring an apoptotic pathway over necrosis for cell death.
PB MDPI
SN 1661-6596
YR 2022
FD 2022-10-28
LK https://hdl.handle.net/10347/43956
UL https://hdl.handle.net/10347/43956
LA eng
NO Arellano-Galindo, L.; Villar-Alvarez, E.; Varela, A.; Figueroa, V.; Fernandez-Vega, J.; Cambón, A.; Prieto, G.; Barbosa, S.; Taboada, P. Hybrid Gold Nanorod-Based Nanoplatform with Chemo and Photothermal Activities for Bimodal Cancer Therapy. Int. J. Mol. Sci. 2022, 23, 13109. https://doi.org/10.3390/ijms232113109
DS Minerva
RD 3 may 2026