RT Journal Article
T1 Characterization of a monoclonal antibody directed against mytilus spp larvae reveals an antigen involved in shell biomineralization
A1 Calvo-Iglesias, Juan
A1 Pérez-Estévez, Daniel
A1 Lorenzo-Abalde, Silvia
A1 Sánchez Correa, Beatriz
A1 Quiroga Berdeal, María Isabel
A1 Fuentes, José M.
A1 González Fernández, África
K1 Hemocytes
K1 Mussels
K1 Larvae
K1 Epithelium
K1 Flow cytometry
K1 Antigen processing and recognition
K1 Immunohistochemistry techniques
K1 Biomineralization
AB The M22.8 monoclonal antibody (mAb) developed against an antigen expressed at the mussel larval and postlarval stages of Mytilus galloprovincialis was studied on adult samples. Antigenic characterization by Western blot showed that the antigen MSP22.8 has a restricted distribution that includes mantle edge tissue, extrapallial fluid, extrapallial fluid hemocytes, and the shell organic matrix of adult samples. Other tissues such as central mantle, gonadal tissue, digestive gland, labial palps, foot, and byssal retractor muscle did not express the antigen. Immunohistochemistry assays identified MSP22.8 in cells located in the outer fold epithelium of the mantle edge up to the pallial line. Flow cytometry analysis showed that hemocytes from the extrapallial fluid also contain the antigen intracellularly. Furthermore, hemocytes from hemolymph have the ability to internalize the antigen when exposed to a cell-free extrapallial fluid solution. Our findings indicate that hemocytes could play an important role in the biomineralization process and, as a consequence, they have been included in a model of shell formation. This is the first report concerning a protein secreted by the mantle edge into the extrapallial space and how it becomes part of the shell matrix framework in M. galloprovincialis mussels.
PB Plos
YR 2016
FD 2016-03-23
LK http://hdl.handle.net/10347/15902
UL http://hdl.handle.net/10347/15902
LA eng
NO Calvo-Iglesias J, Pérez-Estévez D, Lorenzo-Abalde S, Sánchez-Correa B, Quiroga MI, et al. (2016) Characterization of a Monoclonal Antibody Directed against Mytilus spp Larvae Reveals an Antigen Involved in Shell Biomineralization. PLOS ONE 11(3): e0152210. https://doi.org/10.1371/journal.pone.0152210
NO This work was supported by the Xunta de Galicia (Grupo con potencial de crecimientoGPC2013-005). The authors also thank the BIOCAPS project (grant agreement no. FP7- 316265) funded by the European Commission under the 7th Framework Programme (FP7/REGPOT-2012-2013.1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
DS Minerva
RD 27 abr 2026