RT Journal Article
T1 Isolation of betanodavirus from farmed turbot Psetta maxima showing no signs of viral encephalopathy and retinopathy
T2 Aislamiento de betanodavirus en rodaballo de cultivo Psetta maxima que no mostraba signos de encefalopatía y retinopatía víricas
A1 Olveira Hermida, José G.
A1 Souto Pereira, Sandra
A1 Pereira Dopazo, Carlos
A1 Bandín Matos, Isabel
K1 Turbot
K1 Betanodavirus
K1 RGNNV
K1 Susceptibility
K1 Clinical disease
K1 Viral replication
AB The isolation of a betanodavirus strain from juvenile turbot showing unspecific symptoms and very low mortality is reported. The presence of an IPNV-type virus was also revealed in the fish tissues, but only after nested-PCR, suggesting a very low viral load. In addition, different Vibrio species were isolated from some of the individuals. Both viral RNAs were sequenced and phylogenetic analysis indicated that the strain showed 99% identity with the RGNNV genotype. Experimental infections (via immersion and intraperitoneal route) were conducted in order to determine the susceptibility of turbot juveniles (2 and 5 g) to the NNV isolate at 15 and 18 °C (range of turbot rearing temperatures in our area). The results obtained indicated that although the viral isolate was able to replicate in the turbot tissues, it did not induce clinical disease in this fish species. These findings suggest that the existence of a reservoir of NNV-RGNNV type in wild fish in the area represents a low risk for the turbot farming industry
PB Elsevier
YR 2013
FD 2013-05-15
LK https://hdl.handle.net/10347/46009
UL https://hdl.handle.net/10347/46009
LA eng
NO Olveira JG, Souto S, Dopazo CP, Bandín I. Isolation of betanodavirus from farmed turbot Psetta maxima showing no signs of viral encephalopathy and retinopathy. Aquaculture. 2013 Aug 25;406:125-30
NO This work was supported by grants AGL2009-11915 from the Ministerio de Ciencia e Innovación (MICINN) (Spain) and EM 2012/005 from the Consellería de Educación, Cultura e Ordenación Universitaria (Xunta de Galicia) (Spain).
DS Minerva
RD 24 abr 2026