RT Journal Article
T1 Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimp Aristeus antennatus
A1 Heras, Sandra
A1 Planella, Laia
A1 Caldarazzo, Ilaria
A1 Vera Rodríguez, Manuel
A1 García Marín, José Luis
A1 Roldán, María Inés
K1 Aristeus antennatus
K1 Next-generation sequencing
K1 Microsatellite loci
K1 Population genetics
K1 Genetic stocks
K1 Blue and red shrimp
AB The blue and red shrimp, Aristeus antennatus, is a commercially important crustacean, in the Mediterranean Sea, which has been listed as a priority species for fishery management. Hypervariable microsatellite markers could be a useful tool to identify genetic stocks among geographically close fishing grounds. Potential microsatellite markers (97) identified from next-generation sequencing of an individual shrimp using a 454 GS Junior Pyrosequencer were tested on a preliminary panel of 15 individuals representing the four worldwide genetic stocks of the species from which 35 polymorphic loci were identified and used to characterize an additional 20 individuals from the Western Mediterranean Sea. In the Western Mediterranean sample, 32 out of 35 were polymorphic loci and the number of alleles per locus ranged from 2 to 14 and expected heterozygosity ranged from 0.050 to 0.968. No linkage disequilibrium was detected, indicating the independence of the loci. These novel microsatellites provide additional tools to address questions relating to genetic diversity, parentage studies and connectivity patterns of A. antennatus populations and help develop effective strategies to ensure long-term sustainability of this resource
PB PeerJ Inc.
YR 2016
FD 2016
LK http://hdl.handle.net/10347/26111
UL http://hdl.handle.net/10347/26111
LA eng
NO Heras S, Planella L, Caldarazzo I, Vera M, García-Marín J, Roldán MI. 2016. Development and characterization of novel microsatellite markers by Next Generation Sequencing for the blue and red shrimp Aristeus antennatus. PeerJ 4:e2200 https://doi.org/10.7717/peerj.2200
NO This work was supported by grants by the Spanish Government (AGL2009-09228 and CTM2014-54648-C2-2-R) and Ajuntament de Palamós, Spain (Beca Coopoerativa La Equitativa 2013–2014) to MIR. LP benefited from predoctoral fellowship from the Universitat de Girona (BR2014), Spain. IC benefited from a 6 months fellowship from Regione Toscana, Italy, during her stay in Girona
DS Minerva
RD 22 abr 2026