RT Journal Article
T1 Metaproteomic insights into salinity’s impact on carbon assimilation and polyhydroxyalkanoate production in mixed cultures of purple phototrophic bacteria
A1 Lorenzo Llarena, José Ramón
A1 Trueba Santiso, Alba María
A1 Mosquera Corral, Anuska
A1 Pedrouso Fuentes, Alba
K1 Bioreactor
K1 Metabolic pathways
K1 Microbial community adaptation
K1 Photofermentation
K1 Purple non-sulfur bacteria
K1 Osmotic stress
K1 Resource recovery
AB Purple phototrophic bacteria (PPB) are metabolically versatile microorganisms capable of adapting to diverse environmental conditions in biotechnological applications, including polyhydroxyalkanoate (PHA) production. This study explores how salinity drives metabolic specialization by comparing non-saline (R-NS) and saline-adapted (R-S) cultures fed with acetic acid. R-S accumulated significantly more PHA (up to 42 dw%) than in R-NS (up to 11%). Proteomic analyses revealed distinct communities and carbon assimilation pathways. R-NS, dominated by Rhodopseudomonas, relied on the glyoxylate cycle. While R-S, dominated by Cereibacter, employed the ethylmalonyl-CoA (EMC) pathway. EMC may provide proton motive force to support osmotic regulation in R-S, with excess reducing power equivalents stored as PHA. This is supported by glutamate synthesis overexpression and detection of osmolyte transporters. These findings underscore the adaptability of PPB to salinity, the role of reducing power in saline stress response, and suggest that the EMC pathway can sustain PHA biosynthesis even with carboxylation steps.
PB Elsevier
SN 0960-8524
YR 2025
FD 2025-08-18
LK https://hdl.handle.net/10347/43734
UL https://hdl.handle.net/10347/43734
LA eng
NO Lorenzo-Llarena, J. R., Trueba-Santiso, A., Mosquera-Corral, A., & Pedrouso, A. (2025). Metaproteomic insights into salinity’s impact on carbon assimilation and polyhydroxyalkanoate production in mixed cultures of purple phototrophic bacteria. Bioresource Technology, 438, 133171. 10.1016/j.biortech.2025.133171
NO This work was supported by the Spanish Government (MICIU/AEI/ 10.13039/501100011033) through the ECOPOLYVER (PID2020- 112550RB-C21) project. José Ramón Lorenzo-Llarena is grateful to the Ministry of Science, Innovation, and Universities (Spain) for the FPU Grant (FPU-021/05797). Alba Pedrouso acknowledges the Xunta de Galicia (Spain) for her postdoctoral fellowships (ED481B-2021-041 and ED481D-2024-010). The authors are part of a Galician Competitive Research Group (GRC ED431C2021/37), a program co-funded by FEDER (UE).
DS Minerva
RD 23 abr 2026