RT Journal Article
T1 Contamination and sample mix-up can best explain some patterns of mtDNA instabilities in buccal cells and oral squamous cell carcinoma
A1 Bandelt, Hans-Jürgen
A1 Salas Ellacuriaga, Antonio
AB The study of somatic DNA instabilities constitutes a debatable topic because different causes canlead to seeming DNA alteration patterns between different cells or tissues from the sameindividual. Carcinogenesis or the action of a particular toxic could generate such patterns, and thisis in fact the leitmotif of a number of studies on mitochondrial DNA (mtDNA) instability. Patternsof seeming instabilities could also arise from technical errors at any stage of the analysis (DNAextraction, amplification, mutation screening/sequencing, and documentation). Specifically,inadvertent DNA contamination or sample mixing would yield mosaic variation that could beerroneously interpreted as real mutation differences (instabilities) between tissues from the sameindividual. From the very beginning, mtDNA studies comparing cancerous to non-cancerous tissueshave suffered from such mosaic results. We demonstrate here that the phylogenetic linkage ofwhole arrays of mtDNA mutations provides strong evidence of artificial recombination in previousstudies on buccal cells and oral squamous cell carcinoma.
PB BMC
YR 2009
FD 2009
LK http://hdl.handle.net/10347/22769
UL http://hdl.handle.net/10347/22769
LA eng
NO Bandelt, H., Salas, A. Contamination and sample mix-up can best explain some patterns of mtDNA instabilities in buccal cells and oral squamous cell carcinoma. BMC Cancer 9, 113 (2009). https://doi.org/10.1186/1471-2407-9-113
NO This work was partially supported by grants from the Xunta de Galicia(Grupos Emerxentes; 2008/037), Ministerio de Ciencia e Innovación(SAF2008-02971), and Fundación de Investigación Médica Mutua Madrileña(2008/CL444) given to AS
DS Minerva
RD 22 abr 2026