RT Journal Article T1 Effect of fire factors (smoke, ash, charcoal and heat) on seeds of plant species A1 Cruz de la Fuente, Óscar A1 Riveiro, Sheila F. A1 Casal Jiménez, María Mercedes A1 Reyes Ferreira, Otilia K1 Fire treatments K1 Incubation conditions K1 Scarification methodology K1 Seeds germination K1 Viability test AB The effect of the main fire factors (smoke, ash, charcoal and heat) can influence the germination of species through their seeds. Hence, a methodology has been devised in order to have a common protocol for those who work in this area and serve as a valuable tool to compare different species that can be beneficial or detrimental to the conditions of a forest fire. Moreover, the methodology was completed with a check of the viability of the seeds before starting the germination process and another viability test with the non-germinated seeds (dormant or dead) after carrying out the germination test. In this way, the study of the main factors of fire, in combination with the study of the viability of the seeds, can be very useful to know the reproductive behaviour of herbaceous, shrub and tree species in a forest fire scenario. In addition, from the data obtained during the germination process of the seeds, a series of explained parameters can be obtained to better interpret the data acquired from the laboratory experiment and subsequent comparison. For all these reasons, the study of the germination of species in laboratory conditions can help us understand how they behave in the field PB Elsevier YR 2022 FD 2022 LK http://hdl.handle.net/10347/29061 UL http://hdl.handle.net/10347/29061 LA eng NO MethodsX 9 (2022) 101679 NO This work was supported by the Spanish Ministry of Science and Innovation, the Galicia Regional Government, the European Regional Development Fund (ERDF) and Castilla y León Regional Government in the framework of the FIRESEVES (AGL2017–86075-C2–2-R) and WUIFIRECYL (LE005P20) Projects and the Competitive Reference Group BIOAPLIC (ED431C 2019/07) and the Strategic Researcher Cluster BioReDeS (ED431E 2018/09) DS Minerva RD 27 abr 2026