RT Journal Article
T1 The coagulation system helps control infection caused by the ciliate parasite Philasterides dicentrarchi in the turbot Scophthalmus maximus (L.)
A1 Blanco Abad, Verónica
A1 Noia Guldrís, Manuel
A1 Valle Cao, Alejandra
A1 Fontenla Iglesias, Francisco
A1 Folgueira López, Iria
A1 Felipe González, Ana Paula de
A1 Pereiro González, Patricia
A1 Leiro Vidal, José Manuel
A1 Lamas Fernández, Jesús
K1 Fish
K1 Philasterides dicentrarchi
K1 Coagulation system
K1 Neutrophils
K1 Plasma
K1 Complement
AB Many studies have shown that coagulation systems play an important role in the defence against pathogens in invertebrates and vertebrates. In vertebrates, particularly in mammals, it has been established that the coagulation system participates in the entrapment of pathogens and activation of the early immune response. However, functional studies investigating the importance of the fish coagulation system in host defence against pathogens are scarce. In the present study, injection of turbot (Scopthalamus maximus) with the pathogenic ciliate Philasterides dicentrarchi led to the formation of macroscopic intraperitoneal clots in the fish. The clots contained abundant, immobilized ciliates, many of which were lysed. We demonstrated that the plasma clots immobilize and kill the ciliates in vitro. To test the importance of plasma clotting in ciliate killing, we inhibited the process by adding a tetrapeptide known to inhibit fibrinogen/thrombin clotting in mammals. Plasma tended to kill P. dicentrarchi slightly faster when clotting was inhibited by the tetrapeptide, although the total mortality of ciliates was similar. We also found that kaolin, a particulate activator of the intrinsic pathway in mammals, accelerates plasma clotting in turbot. In addition, PMA-stimulated neutrophils, living ciliates and several ciliate components such as cilia, proteases and DNA also displayed procoagulant activity in vitro. Injection of fish with the ciliates generated the massive release of neutrophils to the peritoneal cavity, with formation of large aggregates in those fish with live ciliates in the peritoneum. We observed, by SEM, numerous fibrin-like fibres in the peritoneal exudate, many of which were associated with peritoneal leukocytes and ciliates. Expression of the CD18/CD11b gene, an integrin associated with cell adhesion and the induction of fibrin formation, was upregulated in the peritoneal leukocytes. In conclusion, the findings of the present study show that P. dicentrarchi induces the formation of plasma clots and that the fish coagulation system may play an important role in immobilizing and killing this parasite
PB Elsevier
SN 0145-305X
YR 2018
FD 2018-06-20
LK http://hdl.handle.net/10347/18167
UL http://hdl.handle.net/10347/18167
LA eng
NO Blanco-Abad V, Noia M, Valle A, Fontenla F, Folgueira I, De Felipe AP, Pereiro P, Leiro J, Lamas J. (2018). The coagulation system helps control infection caused by the ciliate parasite Philasterides dicentrarchi in the turbot Scophthalmus maximus (L.). Dev Comp Immunol. 87:147-156. doi: 10.1016/j.dci.2018.06.001
NO This is the accepted manuscript of the following article: Blanco-Abad, V., Noia, M., Valle, A., Fontenla, F., Folgueira, I., & De Felipe, A. et al. (2018). The coagulation system helps control infection caused by the ciliateparasite Philasterides dicentrarchi in the turbot Scophthalmus maximus (L.). Developmental & Comparative Immunology, 87, 147-156. doi: 10.1016/j.dci.2018.06.001
NO This work was financially supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 634429 (PARAFISHCONTROL), by the Ministerio de Economía y Competitividad  (Spain) under grant agreement AGL2014-57125-R and by grant GPC2014/069 from the Xunta de Galicia (Spain)
DS Minerva
RD 28 abr 2026