RT Journal Article
T1 A foundation for comparative genomics and evolutionary studies in Nucella lapillus based on complete mitogenome assembly
A1 García Souto, Daniel
A1 Fernández Rodríguez, Jonathan
A1 Vidal Capón, André
A1 Fuller, Neil
A1 Pizcueta Leirós, Juan
A1 Pasantes Ludeña, Juan José
A1 Galindo Dasilva, Juan
K1 Atlantic dog whelk
K1 Nucella lapillus
K1 Mitochondrial genome
K1 Comparative phylogenomics
K1 Divergent  evolution
K1 Genetic diversity
AB The Atlantic dog whelk, Nucella lapillus, is a marine snail that exhibits divergent evolution in response to habitat adaptation, resulting in distinct populations at the phenotypic, genotypic, and karyotypic levels. In this study, we utilized short- and long-read NGS data to perform a de novo assembly of the entire mitochondrial genome of N. lapillus and developed a multiplex PCR protocol to sequence most of its length using ONT sequencing. Our analysis revealed a typical circular configuration of 16,474 bp in length with 13 protein-coding genes, 22 different tRNA genes, 2 of them showing two copies, 2 rRNA genes, and a control region. Long-read sequencing enabled us to identify a 1826 bp perfect inverted repeat within the control region. Comparative analysis of the mitogenomes of related species in the Muricidae family revealed a conserved gene configuration for N. lapillus. We found a low genetic diversity, as well as a moderate genetic differentiation among the studied populations. Interestingly, there was no observed differentiation between the two chromosomal races, suggesting either introgression and permanent incorporation of the mitochondrial DNA haplotype from one of the chromosomal races into the other or a slower evolutionary rate of the mtDNAs with respect to that of the karyotypes. Our study serves as a foundation for comparative genomics and evolutionary investigations in this species
PB Springer
SN 0025-3162
YR 2024
FD 2024-04-04
LK http://hdl.handle.net/10347/34900
UL http://hdl.handle.net/10347/34900
LA eng
NO Mar Biol 171, 111 (2024)
NO DG-S acknowledges support from the Consellería de Cultura, Educación e Ordenación 361 Universitaria, Xunta de Galicia, under the grant ED481D-2022-001. Funding was provided by the Ministerio de Ciencia e Innovación (PID2021-124930NB-I00) to JG, the ASSEMBLE PLUS (5th 363 call) programme to DG-S (NLA Chrom Evol), Xunta de Galicia (Centro singular de investigación de Galicia accreditation 2019-2022), the European Union (European Regional Development Fund - ERDF) and Xunta de Galicia (ED431C 2020-05)
DS Minerva
RD 28 abr 2026