RT Journal Article
T1 Supercritical CO2 technology for one-pot foaming and sterilization of polymeric scaffolds for bone regeneration
A1 Santos Rosales, Víctor
A1 Magariños Ferro, Beatriz
A1 Starbird-Perez, Ricardo
A1 Suárez González, Javier
A1 Fariña, José B.
A1 Álvarez Lorenzo, Carmen
A1 García González, Carlos A.
K1 Sterilization
K1 Spores
K1 Supercritical CO2
K1 Hydrogen peroxide
K1 Bone scaffold
AB Sterilization is a quite challenging step in the development of novel polymeric scaffolds for regenerative medicine since conventional sterilization techniques may significantly alter their morphological and physicochemical properties. Supercritical (sc) sterilization, i.e. the use of scCO2 as a sterilizing agent, emerges as a promising sterilization method due to the mild operational conditions and excellent penetration capability. In this work, a scCO2 protocol was implemented for the one-pot preparation and sterilization of poly(-caprolactone) (PCL)/poly(lactic-co-glycolic acid) (PLGA) scaffolds. The sterilization conditions were established after screening against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) vegetative bacteria and spores of Bacillus stearothermophilus, Bacillus pumilus and Bacillus atrophaeus. The transition from the sterilization conditions (140 bar, 39 °C) to the compressed foaming (60 bar, 26 °C) was performed through controlled depressurization (3.2 bar/min) and CO2 liquid flow. Controlled depressurization/pressurization cycles were subsequently applied. Using this scCO2 technology toolbox, sterile scaffolds of well-controlled pore architecture were obtained. This sterilization procedure successfully achieved not only SAL-6 against well-known resistant bacteria endospores but also improved the scaffold morphologies compared to standard gamma radiation sterilization procedures
PB Elsevier
SN 0378-5173
YR 2021
FD 2021
LK http://hdl.handle.net/10347/26668
UL http://hdl.handle.net/10347/26668
LA eng
NO International Journal of Pharmaceutics, 605 (2021), 120801. https://doi.org/10.1016/j.ijpharm.2021.120801
NO This work was supported by Xunta de Galicia [ED431F 2016/01, ED431C 2020/17], MCIUN [RTI2018-094131-A-I00], MINECO [SAF2017-83118-R], Consellería de Sanidade, Servizo Galego de Saúde, Axencia de Coñecemento e Saúde (ACIS, CT850A-G), Agencia Estatal de Investigación [AEI] and FEDER funds. V. Santos-Rosales acknowledges to Xunta de Galicia (Consellería de Cultura, Educación e Ordenación Universitaria) for a predoctoral research fellowship [ED481A-2018/014]. C.A. García-González acknowledges to MINECO for a Ramón y Cajal Fellowship [RYC2014-15239]
DS Minerva
RD 29 abr 2026