RT Journal Article
T1 Outer membrane vesicles (OMVs) from Tenacibaculum maritimum as a potential vaccine against fish tenacibaculosis
A1 Escribano Rodríguez, María del Pilar
A1 Balado Dacosta, Miguel
A1 Estévez Toranzo, María Alicia Carolina
A1 Lemos Ramos, Manuel Luis
A1 Magariños Ferro, Beatriz
K1 Tenacibaculum
K1 Tenacibaculosis
K1 Outer membrane vesicles (OMV)
K1 Aquaculture vaccines
K1 Immune response
AB Outer membrane vesicles (OMVs) have been gained increasing attention in vaccinology due to their ability to induce strong protective humoral and cell-mediated immunity. The Gram-negative bacterium Tenacibaculum maritimum, the causative agent of marine tenacibaculosis, poses a significant challenge to the global aquaculture industry due to its difficult prophylaxis. In previous studies, we demonstrated that OMV production is a key virulence mechanism in T. maritimum. Building on this, the present study aimed to evaluate the efficacy of a natural, encapsulated multi-antigen vaccine made from adjuvant-free, crude T. maritimum OMVs (Tm-OMVs). A vaccination experiment using SP9.1-OMVs was conducted in juvenile turbot (Scophthalmus maximus L.), followed by a T. maritimum bath challenge. Immune responses in the turbot were assessed by measuring anti-Tm antibody levels and analyzing the expression of eight key immune-related genes (il-1β, il-8, il-22, pcna, c3, cd4-1, ifng2, cd8α). The results showed that immunization with SP9.1-OMVs provided significant protection against T. maritimum infection (RPS = 70 %). Vaccinated fish exhibited a dose-dependent increase in anti-Tm antibody titers in blood plasma, along with rapid induction of both innate (il-1β, il-8, il-22, c3) and adaptive (cd4-1, ifng2, cd8α) immune genes as early as 4 h post-bath challenge. These findings offer new insights into the early immune response of turbot following T. maritimum infection and could serve as a foundation for developing novel OMV-based vaccines
PB Elsevier
YR 2024
FD 2024-10-05
LK https://hdl.handle.net/10347/40296
UL https://hdl.handle.net/10347/40296
LA eng
NO Escribano MP, Balado M, Santos B, Toranzo AE, Lemos ML, Magariños B. Outer membrane vesicles (OMVs) from Tenacibaculum maritimum as a potential vaccine against fish tenacibaculosis. Fish Shellfish Immunol. 2024 Nov;154:109943. doi: 10.1016/j.fsi.2024.109943. Epub 2024 Oct 5
NO MPE acknowledges Ministerio de Ciencia e Innovación of Spain for a predoctoral Fellowship (FPI Programme Fellowship No. PRE2019-089544
NO This work was also supported by grant ED431C2022/23 from Xunta de Galicia
DS Minerva
RD 25 abr 2026