RT Journal Article
T1 Response of Three Different Viruses to Interferon Priming and Dithiothreitol Treatment of Avian Cells
A1 Lostalé Seijo, Irene
A1 Martínez Costas, José Manuel
A1 Benavente Martínez, Francisco Javier
AB We have previously shown that the replication of avian reovirus (ARV) in chicken cells is much more resistant to interferon(IFN) than the replication of vesicular stomatitis virus (VSV) or vaccinia virus (VV). In this study, we have investigated the rolethat the double-stranded RNA (dsRNA)-activated protein kinase (PKR) plays in the sensitivity of these three viruses toward theantiviral action of chicken interferon. Our data suggest that while interferon priming of avian cells blocks vaccinia virus replication by promoting PKR activation, the replication of vesicular stomatitis virus appears to be blocked at a pretranslational step.Our data further suggest that the replication of avian reovirus in chicken cells is quite resistant to interferon priming becausethis virus uses strategies to downregulate PKR activation and also because translation of avian reovirus mRNAs is more resistantto phosphorylation of the alpha subunit of initiation factor eIF2 than translation of their cellular counterparts. Our results further reveal that the avian reovirus protein sigmaA is able to prevent PKR activation and that this function is dependent on itsdouble-stranded RNA-binding activity. Finally, this study demonstrates that vaccinia virus and avian reovirus, but not vesicularstomatitis virus, express/induce factors that counteract the ability of dithiothreitol to promote eIF2 phosphorylation. Our datademonstrate that each of the three different viruses used in this study elicits distinct responses to interferon and to dithiothreitol-induced eIF2 phosphorylation when infecting avian cells
PB American Society for Microbiology
SN 0022-538X
YR 2016
FD 2016
LK http://hdl.handle.net/10347/18338
UL http://hdl.handle.net/10347/18338
LA eng
NO Lostalé-Seijo I, Martínez-Costas J, Benavente J. 2016. Response of three different viruses to interferon priming and dithiothreitol treatment of avian cells. J Virol 90:8328 –8340. doi:10.1128/JVI.01175-16
NO The Spanish Ministerio de Economía y Competitividad provided fundingto Javier Benavente and José Martínez-Costas under grant numbersBFU2010-22228 and BFU2013-43513-R. Support was also provided bythe Xunta de Galicia (CN 2012/018). Irene Lostalé-Seijo was a recipient ofa predoctoral FPU fellowship (Ministerio de Educación y Ciencia) and aResearch Fellowship (Bolsa de Investigación; Deputación Provincial daCoruña)
DS Minerva
RD 28 abr 2026