RT Journal Article
T1 Bundling the removal of emerging contaminants with the production of ligninolytic enzymes from residual streams
A1 González Rodríguez, Sandra
A1 Chen, Xinyi
A1 Eibes González, Gemma María
A1 Pizzi, Antonio
A1 Feijoo Costa, Gumersindo
A1 Moreira Vilar, María Teresa
A1 Lu Chau, Thelmo Alejandro
K1 Manganese peroxidase
K1 Irpex lacteus
K1 Wheat straw
K1 Emerging contaminants
K1 Enzymatic oxidation
AB Enzymes offer interesting features as biological catalysts for industry: high specificity, activity under mild conditions, accessibility, and environmental friendliness. Being able to produce enzymes in large quantities and having them available in a stable and reusable form reduces the production costs of any enzyme-based process. Agricultural residues have recently demonstrated their potential as substrates to produce ligninolytic enzymes by different white rot fungi. In this study, the biotechnological production of a manganese peroxidase (MnP) by Irpex lacteus was conducted through solid-state fermentation (SSF) with wheat straw as substrate and submerged fermentation (SmF) employing wheat straw extract (WSE). The obtained enzyme cocktail also showed manganese-independent activity (MiP), related to the presence of a short MnP and a dye-decolorizing peroxidase (DyP) which was confirmed by shotgun proteomic analyses. In view of the enhanced production of ligninolytic enzymes in SmF, different parameters such as WSE concentration and nitrogen source were evaluated. The highest enzyme titers were obtained with a medium formulated with glucose and peptone (339 U/L MnP and 15 U/L MiP). The scale-up to a 30 L reactor achieved similar activities, demonstrating the feasibility of enzyme production from the residual substrate at different production scales. Degradation of five emerging pollutants was performed to demonstrate the high oxidative capacity of the enzyme. Complete removal of hormones and bisphenol A was achieved in less than 1 h, whereas almost 30% degradation of carbamazepine was achieved in 24 h, which is a significant improvement compared to previous enzymatic treatments of this compound
PB Springer
SN 0175-7598
YR 2022
FD 2022
LK http://hdl.handle.net/10347/29164
UL http://hdl.handle.net/10347/29164
LA eng
NO Applied Microbiology and Biotechnology (2022) 106:1299–1311.  https://doi.org/10.1007/s00253-022-11776-7
NO Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This research was funded by the Spanish Ministry of Science and Innovation: MODENA Project (CTQ2016-79461-R) and the WooBAdh project (PCI2018-092866, ERA-CoBioTech program)
DS Minerva
RD 23 abr 2026