RT Journal Article
T1 Determination of lamotrigine in human plasma by HPLC-PDA. Application to forensic samples
A1 Sánchez Sellero, Inés
A1 Álvarez Freire, Iván
A1 Cabarcos Fernández, Pamela
A1 Janza Candal, Lidia
A1 Tabernero Duque, María Jesús
A1 Bermejo Barrera, Ana María
K1 Lamotrigine
K1 Plasma
K1 HPLC–PDA
K1 Forensic
AB PurposeTherapeutic drug monitoring of plasma lamotrigine (LTG) has customarily been carried out in order to prevent some its adverse effects. For forensic purposes, determination of LTG in plasma is an useful tool in cases of accidental overdose or suicidal attempts. Currently, there are several analytical methods available including some based on LC tandem mass spectrometry techniques, but simple and accessible LC-UV methods still can be useful for the purpose. Here we report on a new high-performance liquid chromatography method for the determination of lamotrigine in human plasma which has been developed and validated including selectivity, sensitivity, accuracy, precision and recovery studies.MethodsLamotrigine and the internal standard chloramphenicol were extracted from plasma using liquid-liquid extraction using small volumes of buffer and ethylacetate. Detection was monitored at 305.7 and 276.0 nm for lamotrigine and chloramphenicol, respectively.ResultsThe method was linear concentration dependence within the range of 0.1–10 µg/ml, with a mean coefficient of correlation r = 0.993. The limit of detection (LOD) was 0.04 µg/ml and the limit of quantification (LOQ) was 0.1 µg/ml. Intra and interday precision values were lower than 9.0% at all concentrations studied. The intra and interday accuracy values ranged from − 7.6 to 10.1%. Recovery was found to be 98.9% or higher. The method here described was successfully applied to 11 postmortem blood samples received at the Forensic Sciences Institute of Santiago de Compostela (Spain).ConclusionA new HPLC method for the determination of lamotrigine in human plasma was developed and validated. A liquid-liquid extraction using small volumes of buffer and ethylacetate was optimized. The proposed method is suitable for forensic toxicological analysis
PB Springer
YR 2024
FD 2024-04-10
LK http://hdl.handle.net/10347/34864
UL http://hdl.handle.net/10347/34864
LA eng
NO Forensic Sci Med Pathol (2024)
DS Minerva
RD 24 abr 2026