RT Journal Article
T1 New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species
A1 Felipe González, Ana Paula de
A1 Lamas Fernández, Jesús
A1 Sueiro Benavides, Rosa Ana
A1 Folgueira López, Iria
A1 Leiro Vidal, José Manuel
K1 Paralichthys adspersus
K1 Scophthalmus maximus
K1 Scuticociliates
K1 SSUrRNA gene
K1 α- β-tubulin gene
AB Scuticociliatosis is a severe disease in farmed flatfish. However, the causative agent is not always accurately identified. In this study, we identified two isolates of scuticociliates from an outbreak in cultured fine flounder Paralichthys adspersus. Scuticociliate identification was based on morphological data, examination of life stages and the use of molecular approaches. The isolates were compared with a strain of Philasterides dicentrachi from turbot Scophthalmus maximus and with a strain deposited in the American Type Culture Collection as Miamiensis avidus ATCC® 50180™. The use of morphological, biological and molecular methods enabled us to identify the isolates from the fine flouder as P. dicentrarchi. Comparison of P. dicentrachi isolates and M. avidus revealed some differences in the buccal apparatus. Unlike P. dicentrarchi, M. avidus has a life cycle with three forms: macrostomes (capable of feeding on P. dicentrarchi), microstomes and tomites. Additionally, we found differences in the 18S rRNA and α- and β-tubulin gene sequences, indicating that P. dicentrarchi and M. avidus are different species. We therefore reject the synonymy/conspecificity of the two taxa previously suggested. Finally, we suggest that a combination of morphological, biological, molecular (by multigene analysis) and serological techniques could improve the identification of scuticociliates parasites in fish
PB Cambridge University Press
SN 0031-1820
YR 2017
FD 2017-09
LK http://hdl.handle.net/10347/18170
UL http://hdl.handle.net/10347/18170
LA eng
NO DE Felipe AP, Lamas J, Sueiro RA, Folgueira I, Leiro JM. (2017). New data on flatfish scuticociliatosis reveal that Miamiensis avidus and Philasterides dicentrarchi are different species. Parasitology 29:1-18. doi: 10.1017/S0031182017000749
NO This article has been published in a revised form in Parasitology [http://doi.org/10.1017/S0031182017000749]. This version is free to viewand download for private research and study only. Not for re-distribution,re-sale or use in derivative works. © 2017 Cambridge University Press
NO This work was financially supported by the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 634429 (PARAFISHCONTROL), by the Ministerio de Economía y Competitividad  (Spain) under grant agreement AGL2014-57125-R and by grant GPC2014/069 from the Xunta de Galicia (Spain)
DS Minerva
RD 24 abr 2026