RT Journal Article
T1 iTRAQ-based proteomic analysis reveals potential serum biomarkers of allergic and nonallergic asthma
T2 Serum biomarkers of allergic and non-allergic asthma
A1 Nieto Fontarigo, Juan José
A1 González Barcala, Francisco Javier
A1 Andrade-Bulos, Luis Juan
A1 San-José, María Esther
A1 Cruz, María Jesús
A1 Valdés Cuadrado, Luis
A1 Crujeiras Casais, Rosa María
A1 Arias Crespo, María del Pilar
A1 Salgado Castro, Francisco Javier
K1 Allergic asthma
K1 Asthma biomarker
K1 IGFALS
K1 Nonallergic asthma
K1 Serum proteomics
AB Background: Asthma is heterogeneous disease with different phenotypes, endotypes and severities. Definition of these subgroups requires the identification of biomarkers in biological samples, and serum proteomics is a useful and minimally invasive method for this purpose. Therefore, the aim of this study was to detect serum proteins whose abundance is distinctively associated with different asthma phenotypes(allergic vs nonallergic) or severities.Methods: For each group of donors (32 healthy controls, 43 allergic rhinitis patients and 192 asthmatics with different phenotypes and severities), we generated two pools of sera that were analysed by a shotgun MS approach based on combinatorial peptide ligand libraries and iTRAQ-LC-MS/MS.Results: MS analyses identified 18 proteins with a differential abundance. Functional/network study of these proteins identified key processes for asthma pathogenesis, such as complement activation, extracellular matrix organization, platelet activation and degranulation, or post-translational protein phosphorylation. Furthermore, our results highlighted an enrichment of the “Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)” route in allergic asthma and the lectin pathway of complement activation in nonallergic asthma.
PB Wiley (on behalf of European Academy of Allergy and Clinical Immunology)
SN 0105-4538
YR 2020
FD 2020-12-01
LK https://hdl.handle.net/10347/39340
UL https://hdl.handle.net/10347/39340
LA eng
NO Nieto-Fontarigo JJ, González-Barcala FJ, Andrade-Bulos LJ, et al. iTRAQ-based proteomic analysis reveals potential serum biomarkers of allergic and nonallergic asthma. Allergy. 2020;75:3171–3183. https://doi.org/10.1111/ all.14406
NO This is the peer reviewed version of the following article: Nieto-Fontarigo JJ, González-Barcala FJ, Andrade-Bulos LJ, et al. iTRAQ-based proteomic analysis reveals potential serum biomarkers of allergic and nonallergic asthma. Allergy. 2020;75:3171–3183, which has been published in final form at https://doi.org/10.1111/ all.14406. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.
NO Sociedad Española de Neumología y Cirugía Torácica (SEPAR) (121/2012)
NO This study was funded by different grants: a) Sociedad Española de NeumologInstituto de Salud Carlos III, Ministerio de Economía y Competitividad (Fondo de Investigación Sanitaria, FIS, co-funded by European Union ERDF funds) (PI13/02046)
NO Xunta de Galicia PhD Fellowship (Co-funded by European Social Fund (ESF) (JJNF)
DS Minerva
RD 23 abr 2026