RT Journal Article
T1 Successful hydraulic strategies to start up OLAND sequencing batch reactors at lab scale
A1 Schaubroeck, Thomas
A1 Bagchi, Samik
A1 De Clippeleir, Haydée
A1 Carballa Arcos, Marta
A1 Verstraete, Willy
A1 Vlaeminck, Siegfried E.
AB Oxygen‐limited autotrophic nitrification/denitrification (OLAND) is a one‐stage combination of partial nitritation and anammox, which can have a challenging process start‐up. In this study, start‐up strategies were tested for sequencing batch reactors (SBR), varying hydraulic parameters, i.e. volumetric exchange ratio (VER) and feeding regime, and salinity. Two sequential tests with two parallel SBR were performed, and stable removal rates > 0.4 g N l−1 day−1 with minimal nitrite and nitrate accumulation were considered a successful start‐up. SBR A and B were operated at 50% VER with 3 g NaCl l−1 in the influent, and the influent was fed over 8% and 82% of the cycle time respectively. SBR B started up in 24 days, but SBR A achieved no start‐up in 39 days. SBR C and D were fed over 65% of the cycle time at 25% VER, and salt was added only to the influent of SBR D (5 g NaCl l−1). Start‐up of both SBR C and D was successful in 9 and 32 days respectively. Reactor D developed a higher proportion of small aggregates (0.10–0.25 mm), with a high nitritation to anammox rate ratio, likely the cause of the observed nitrite accumulation. The latter was overcome by temporarily including an anoxic period at the end of the reaction phase. All systems achieved granulation and similar biomass‐specific nitrogen removal rates (141–220 mg N g−1 VSS day−1). FISH revealed a close juxtapositioning of aerobic and anoxic ammonium‐oxidizing bacteria (AerAOB and AnAOB), also in small aggregates. DGGE showed that AerAOB communities had a lower evenness than Planctomycetes communities. A higher richness of the latter seemed to be correlated with better reactor performance. Overall, the fast start‐up of SBR B, C and D suggests that stable hydraulic conditions are beneficial for OLAND while increased salinity at the tested levels is not needed for good reactor performance
PB Wiley
YR 2012
FD 2012
LK http://hdl.handle.net/10347/21890
UL http://hdl.handle.net/10347/21890
LA eng
NO Schaubroeck, T., Bagchi, S., De Clippeleir, H., Carballa, M., Verstraete, W. and Vlaeminck, S.E. (2012), Successful hydraulic strategies to start up OLAND sequencing batch reactors at lab scale. Microbial Biotechnology, 5: 403-414. doi:10.1111/j.1751-7915.2011.00326.x
NO S.E.V. was supported as a postdoctoral fellow from the Research Foundation Flanders (FWO‐Vlaanderen), S.B. was supported by a scholarship from the Flemish Government (1F2B8M/JDW/2010–2011/10‐BTL‐IND‐01) on bilateral cultural cooperation programme, H.D.C. was recipient of a PhD grant from the Institute for the Promotion of Innovation by Science and Technology in Flanders (IWT‐Vlaanderen, number SB‐81068), and M.C. was supported by a postdoctoral contract from the Xunta de Galicia (Isidro Parga Pondal program, IPP‐08–37)
DS Minerva
RD 23 abr 2026