RT Journal Article
T1 Magnetic-driven Interleukin-4 internalization promotes magnetic nanoparticle morphology and size-dependent macrophage polarization
A1 Arnosa Prieto, Ángela
A1 Díaz Rodríguez, Patricia
A1 González Gómez, Manuel Antonio
A1 García Acevedo, Pelayo
A1 Alves, Lisandra Cristina de Castro
A1 Piñeiro Redondo, Yolanda
A1 Rivas Rey, José
K1 Magnetic nanoparticle
K1 Iron oxide
K1 Interleukin-4
K1 Macrophage polarization
K1 M1
K1 M2
AB Macrophages are known to depict two major phenotypes: classically activated macrophages (M1), associated with high production of pro-inflammatory cytokines, and alternatively activated macrophages (M2), which present an anti-inflammatory function. A precise control over M1-M2 polarization is a promising strategy in therapeutics to modulate both tissue regeneration and tumor progression processes. However, this is not a simple task as macrophages behave differently depending on the microenvironment. In agreement with this, non-consistent data have been reported regarding macrophages response to magnetic iron oxide nanoparticles (MNPs). To investigate the impact of both tissue microenvironment and MNPs properties on the obtained macrophage responses, single-core (SC) and multi core (MC) citrate coated MNPs, are synthesized and, afterwards, loaded with a macrophage polarization trigger, IL-4. The developed MNPs are then tested in macrophages subjected to different stimuli. We demonstrate that macrophages treated with low concentrations of MNPs behave differently depending on the polarization stage independently of the concentration of iron. Moreover, we find out that MNPs size and morphology determines the effect of the IL-4 loaded MNPs on M1 macrophages, since IL-4 loaded SC MNPs favor the polarization of M1 macrophages towards M2 phenotype, while IL-4 loaded MC MNPs further stimulate the secretion of pro-inflammatory cytokines
PB Elsevier
SN 0021-9797
YR 2024
FD 2024
LK http://hdl.handle.net/10347/32915
UL http://hdl.handle.net/10347/32915
LA eng
NO Journal of Colloid and Interface Science 655 (2024) 286-295
NO This work was supported by the Programa de Axudas de apoio á etapa predoutoral 2020 of Xunta de Galicia, by the European Commission under the BOW project (FETPROACT-EIC-05-2019, Grant 952183), CARTsol project (PLEC2022-009217 funded by MICINN/AEI /10.13039/501100011033 and NextGenerationEU/ PRTR) and partially supported by the Spanish Ministry of Science and Innovation (ref PID2020-112626RB-C21), Modalities «Research Challenges» and «Knowledge Generation» and the Regional Consellería de Innovación Program for the Grupos de Referencia Competitiva 2021 —GRC2021 project of Xunta de Galicia
DS Minerva
RD 23 abr 2026