RT Journal Article
T1 High Prevalence and Diversity of Cephalosporin-Resistant Enterobacteriaceae Including Extraintestinal Pathogenic E. coli CC648 Lineage in Rural and Urban Dogs in Northwest Spain
A1 Abreu Salinas, Fátima
A1 Díaz Jiménez, Dafne
A1 García Meniño, Isidro
A1 Lumbreras, Pilar
A1 López Beceiro, Ana María
A1 Fidalgo Álvarez, Luis Eusebio
A1 Rodicio Rodicio, María del Rosario
A1 Mora Gutiérrez, Azucena
A1 Fernández, Javier
K1 ST1485 (CC648)
K1 ExPEC
K1 MDR
K1 ESBL
K1 AmpC
K1 Dogs
K1 E. coli
K1 K. pneumoniae
AB The aim of this work was to assess the prevalence of extended spectrum-β-lactamase (ESBL)- and carbapenemase-producing Enterobacteriaceae in fecal samples recovered from rural and urban healthy dogs in Northwest Spain (Galicia) to identify potential high-risk clones and to molecularly characterize positive isolates regarding the genes coding for ESBL/pAmpC resistance and virulence. Thirty-five (19.6%) out of 179 dogs were positive for cephalosporin-resistant Enterobacteriaceae, including Escherichiacoli and Klebsiella pneumoniae (39 and three isolates, respectively). All the isolates were multidrug resistant, with high rates of resistance to different drugs, including ciprofloxacin (71.4%). A wide diversity of ESBL/pAmpC enzymes, as well as E. coli phylogroups (A, B1, C, D, E, F and clade I) were found. The eight isolates (20.5%) found to conform to the ExPEC status, belonged to clones O1:H45-clade I-ST770 (CH11-552), O18:H11-A-ST93-CC168 (CH11-neg), O23:H16-B1-ST453-CC86 (CH6-31), and O83:H42-F-ST1485-CC648 (CH231-58), with the latter also complying the uropathogenic (UPEC) status. The three K. pneumoniae recovered produced CTX-M-15 and belonged to the ST307, a clone previously reported in human clinical isolates. Our study highlights the potential role of both rural and urban dogs as a reservoir of high-risk Enterobacteriaceae clones, such as the CC648 of E. coli and antimicrobial resistance traits. Within a One-Health approach, their surveillance should be a priority in the fight against antimicrobial resistance
PB MDPI
YR 2020
FD 2020
LK http://hdl.handle.net/10347/23829
UL http://hdl.handle.net/10347/23829
LA eng
NO Abreu-Salinas, F.; Díaz-Jiménez, D.; García-Meniño, I.; Lumbreras, P.; López-Beceiro, A.M.; Fidalgo, L.E.; Rodicio, M.R.; Mora, A.; Fernández, J. High Prevalence and Diversity of Cephalosporin-Resistant Enterobacteriaceae Including Extraintestinal Pathogenic E. coli CC648 Lineage in Rural and Urban Dogs in Northwest Spain. Antibiotics 2020, 9, 468
NO This research was funded project FIS PI17-00728 (Fondo de Investigación Sanitaria, Instituto de Salud Carlos III, Ministerio de Economía y Competitividad, Spain), cofunded by the European Regional Development Fund of the European Union: a Way to Making Europe (FEDER); Project PID2019-104439RB-C21/AEI/10.13039/501100011033 and FEDER; ED431C 2017/57 from the Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia and FEDER; and by the Strategic Researcher Cluster BioReDeS funded by the Regional Government Xunta de Galicia under the project no. ED431E 2018/09. D. Díaz-Jiménez and I. García-Meniño acknowledge the Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia for their pre-doctoral grants (ED481A-2019/022 and ED481A-2015/149, respectively). The Research stay of I. García-Meniño at the Hospital Universitario Central de Asturias was funded by a grant from the Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC)
DS Minerva
RD 30 abr 2026