RT Journal Article
T1 Lipid nanoparticles as nano-Trojan-horses for siRNA delivery and gene-knockdown
A1 Sánchez-Arribas, Natalia
A1 Velasco Rodríguez, Brenda
A1 Aicart, Emilio
A1 Guerrero-Martínez, Andrés
A1 Junquera, Elena
A1 Taboada Antelo, Pablo
K1 Lipid-based nanovector
K1 Lipid nanoparticles
K1 Gene knockdown
K1 siRNA
K1 Internalization route
K1 Reactive oxygen species
K1 3D cell spheroids
K1 Cytotoxicity
K1 Lamellar phase
AB The therapeutic messenger RNA strategies, such as those using small interfering RNAs, take several advantages (versatility, efficiency and selectivity) over plasmid DNA-based strategies. However, the challenge remains to f ind nanovectors capable of properly loading the genetic material, transporting it through troublesome environments, like a tumoral site, and delivering it into the cytoplasm of target cells. Here, lipid nanoparticles, consisting of a gemini cationic/neutral helper lipid mixture, are proposed as siRNA nanovector. Cells from cervical and brain cancer overexpressing the green fluorescent protein (GFP) were chosen to analyse the biological response as well as the efficiency and safety of the siRNA-loaded nanovector according to the cell phenotype. Flow cytometry and epifluorescence or confocal microscopy were used to follow the gene knockdown in these overexpressed cells. The effect of the nanovector on cellular proliferation was evaluated with cytotoxicity assays while their potential oxidative stress generation was determined by quantifying the generation of reactive oxygen species. To explore the mechanism of cellular uptake, different inhibitors of endocytic pathways were used during incubation with cells. Finally, nanovectors were incubated in 3D-grown cells (spheroids) to see
PB Elsevier
SN 0021-9797
YR 2025
FD 2025
LK https://hdl.handle.net/10347/40797
UL https://hdl.handle.net/10347/40797
LA eng
NO Sánchez-Arribas, N., Rodríguez, B. V., Aicart, E., Guerrero-Martínez, A., Junquera, E., Taboada, P. (2025). Lipid nanoparticles as nano-Trojan-horses for siRNA delivery and gene-knockdown." Journal Of Colloid And Interface Science", vol. 679, 975-987
NO P.T. thanks Agencia Estatal de Investigación (AEI) for funding through project PID2022-142682OB-I00 and PCI2022-134981-2, and Xunta de Galicia for grant ED431C 2022/28. ERDF funds are also acknowledged; E.J. thanks Agencia Estatal de Investigación (AEI) for funding through project PID2021-123228NB-I00
DS Minerva
RD 23 abr 2026