RT Journal Article
T1 Conservation of Zebrafish MicroRNA-145 and Its Role during Neural Crest Cell Development
A1 Steeman, Tomás José
A1 Rubiolo Gaytán, Juan Andrés
A1 Sánchez Piñón, Laura
A1 Calcaterra, Nora B.
A1 Weiner, Andrea
K1 MicroRNA
K1 Neural crest
K1 Gene regulatory network
K1 Embryonic development
AB The neural crest is a multipotent cell population that develops from the dorsal neural fold of vertebrate embryos in order to migrate extensively and differentiate into a variety of tissues. A number of gene regulatory networks coordinating neural crest cell specification and differentiation have been extensively studied to date. Although several publications suggest a common role for microRNA-145 (miR-145) in molecular reprogramming for cell cycle regulation and/or cellular differentiation, little is known about its role during in vivo cranial neural crest development. By modifying miR-145 levels in zebrafish embryos, abnormal craniofacial development and aberrant pigmentation phenotypes were detected. By whole-mount in situ hybridization, changes in expression patterns of col2a1a and Sry-related HMG box (Sox) transcription factors sox9a and sox9b were observed in overexpressed miR-145 embryos. In agreement, zebrafish sox9b expression was downregulated by miR-145 overexpression. In silico and in vivo analysis of the sox9b 3′UTR revealed a conserved potential miR-145 binding site likely involved in its post-transcriptional regulation. Based on these findings, we speculate that miR-145 participates in the gene regulatory network governing zebrafish chondrocyte differentiation by controlling sox9b expression
PB MDPI
YR 2021
FD 2021
LK http://hdl.handle.net/10347/26653
UL http://hdl.handle.net/10347/26653
LA eng
NO Genes 2021, 12(7), 1023; https://doi.org/10.3390/genes12071023
NO This research was funded by a CONICET External Grant (March 2018 to A.M.J.W.), an ANPCyT PICT Grant (PICT-2017-0509 to A.M.J.W.), and a CONICET PIP Grant (PIP-2015-0719 to N.B.C.)
DS Minerva
RD 22 abr 2026