RT Journal Article
T1 Development of a transcriptomic protocol combining bovine somatic cells and OpenArray® technology to trace the ab(use) of somatotropin in dairy cattle
A1 Lamas Freire, Alexandre
A1 Regal López, Patricia
A1 Vázquez Belda, Beatriz Isabel
A1 Cepeda Sáez, Alberto
A1 Franco Abuín, Carlos Manuel
K1 Somatotropin
K1 Somatic cell
K1 Milk
K1 Transcriptomic
AB In the case of recombinant bovine somatotropin (rbST), in Europe it is key to control misuse in cattle in vivo, during the period of lactation of the cow. Therefore, target samples have to be easy to collect, the method of collection should be non-invasive and it has to be economically viable. The goal of this study was to develop a transcriptomic protocol based in the combination of High-Throughput Real-Time PCR and milk somatic cells to detect the ab(use) of rbST in dairy cattle. The results showed that MSCs are an optimal alternative to isolate RNA with good quality using a simple protocol for their collection. The use of OpenArray® technology made possible to establish a transcriptomic profile of treated and control cows that could be used to detect the illegal use of rBST in dairy cattle in EU
PB Proteomass Scientific Society
SN 2182-0287
YR 2017
FD 2017-12
LK http://hdl.handle.net/10347/16237
UL http://hdl.handle.net/10347/16237
LA eng
NO Lamas, A., Regal, P., Vázquez, B., Cepeda, A., Franco, C.M., (2017). Development of a transcriptomic protocol combining bovine somatic cells and OpenArray® technology to trace the ab(use) of somatotropin in dairy cattle. “Journal of Integrated Omics, a methodological journal”, vol. 7 (Special Issue: Book of Abstracts ISPROF 2017), 20
NO SPECIAL ISSUE: SELECTED ABSTRACTS OF THE III INTERNATIONAL CAPARICA SYMPOSIUM ON PROFILING (ISPROF 2017),Costa de Caparica, Portugal, 4th-7th September 2017
NO Ministerio de Economía y Competitividad. Programa Estatal de Investigación, Desarrollo e Innovación Orientada a los Retos de la Sociedad (proyecto AGL2014-58881-R)
DS Minerva
RD 22 abr 2026