RT Journal Article
T1 Feasible microbial accumulation of triacylglycerides from crude glycerol
A1 Fra Vázquez, Andrea
A1 Pedrouso Fuentes, Alba
A1 Palmeiro Sánchez, Tania María
A1 Moralejo Gárate, Helena
A1 Mosquera Corral, Anuska
K1 Bacteria
K1 Bioprocesses
K1 Lipids
K1 Recovery
K1 Waste treatment
K1 Yeast
AB BACKGROUND: Crude glycerol, a by-product of the biodiesel production industry, was used to produce intracellular storage polymers for waste valorization. The enrichment of a mixed microbial culture (MMC) in microorganisms with the ability to accumulate intracellular polymers was performed in a sequencing batch reactor (SBR) submitted to feast–famine conditions. The effect of different carbon sources in the accumulation of biopolymers was investigated. RESULTS: A MMC enriched in yeast and bacteria was obtained using crude glycerol as feedstock. Accumulation experiments performedwith crude glycerol, synthetic glycerol and synthetic methanol showed the feasibility of theMMCto producedifferent biopolymers. Triacylglyceride (TAG) accumulation up to 46wt% in yeast cellswas promoted by the presence of residual lipids in crude glycerol. However, bacteria fromclass Betaproteobacteria used glycerol mainly to accumulate 28wt% of polyglucose (PG) andmethanol as carbon source for cell growth. CONCLUSIONS: Aswaste valorization, a possible advantage which comes out of the present study is the use of open, non-sterile and non-defined systems to produce TAGs. These TAGs can potentially re-enter the biodiesel production process helping on the maximisation of the feedstock used in this process
PB Wiley
SN 1097-4660
YR 2018
FD 2018-04-16
LK http://hdl.handle.net/10347/17683
UL http://hdl.handle.net/10347/17683
LA eng
NO Fra‐Vázquez, A. , Pedrouso, A. , Palmeiro‐Sánchez, T. , Moralejo‐Gárate, H. and Mosquera‐Corral, A. (2018), Feasible microbial accumulation of triacylglycerides from crude glycerol. J. Chem. Technol. Biotechnol, 93: 2644-2651
NO This is the peer-reviewed version of the following article: Fra-Vázquez, A. , Pedrouso, A. , Palmeiro-Sánchez, T. , Moralejo-Gárate, H. and Mosquera-Corral, A. (2018), Feasible microbial accumulation of triacylglycerides fromcrude glycerol. J. Chem. Technol. Biotechnol, 93: 2644-2651, which has been publishedin final form at https://doi.org/10.1002/jctb.5618. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions
NO This research was supported by the Spanish Government (AEI) through FISHPOL (CTQ2014–55021-R) and GRANDSEA (CTM2014–55397-JIN) projects. The authors belong to the Galician Competitive Research Group GRC ED431C 2017/29 and to the CRETUS Strategic Partnership (AGRUP2015/02). All of these programmes are co-funded by the FEDER (EU)
DS Minerva
RD 24 abr 2026