RT Journal Article
T1 Enzymatic Degradation of Low Soluble Compounds in Monophasic Water:Solvent Reactors. Kinetics and Modeling of Anthracene Degradation by MnP
A1 Eibes González, Gemma María
A1 Moreira Vilar, María Teresa
A1 Feijoo Costa, Gumersindo
A1 Lema Rodicio, Juan Manuel
K1 Enzyme bioreactors
K1 Manganese peroxidase
K1 Anthracene
K1 Miscible solvent
K1 Modeling
AB Polycyclic aromatic hydrocarbons (PAHs) are toxic compounds presenting low water solubility and high hydrophobicity, which greatly hampers their natural biodegradation. The enzymatic degradation of a model compound, anthracene, was evaluated in presence of a miscible solvent for an increased solubility. Manganese peroxidase, a ligninolytic enzyme from white-rot fungi, was used as biocatalyst in a medium containing acetone. The kinetic parameters of the enzymatic degradation of anthracene, obtained from fed-batch experiments, were applied to model the operation of a continuous reactor. Kinetics comprised a Michaelis–Menten equation, modified with an autocatalytic term, assumed to the effect of quinones acting as electron carriers, and a logistic function related to enzyme activity. The continuous reactor has been operated for 108 h, attaining a 90% of anthracene degradation, which demonstrated the feasibility of the system for its application in the removal of poorly soluble compounds. The model of this reactor permitted to predict accurately anthracene degradation in different conditions, such as external addition of anthraquinone and different enzymatic activities. Biotechnol. Bioeng. 2008;100: 619–626.
PB Wiley
SN 0006-3592
YR 2008
FD 2008-07-01
LK https://hdl.handle.net/10347/39251
UL https://hdl.handle.net/10347/39251
LA eng
NO Eibes, G., Moreira, M.T., Feijoo, G. and Lema, J.M. (2008), Enzymatic degradation of low soluble compounds in monophasic water:solvent reactors. Kinetics and modeling of anthracene degradation by MnP. Biotechnol. Bioeng., 100: 619-626. https://doi.org/10.1002/bit.21806
DS Minerva
RD 3 may 2026