RT Journal Article
T1 Kiloniella majae sp. nov., isolated from spider crab (Majabrachydactyla) and pullet carpet shell clam (Venerupis pullastra)
A1 Gerpe, Diego
A1 Buján Gómez, Noemí
A1 Diéguez, Ana L.
A1 Lasa González, Aide
A1 López Romalde, Jesús
K1 Kiloniella majae sp. nov
K1 Maja brachydactyla
K1 Venerupis pullastra
K1 Whole genome sequencing
K1 ANI
AB Ten Gram-negative, rod-shaped and motile bacterial strains were isolated from spider crab (M27.10, M27.11a, F36.1, F36.4, M56.1, F76.17b, M146.1, M166.3 and M166.6) and pullet carpet shell clam (SBRF 1.10) collected in the coast of Galicia. Analyses of the 16S rRNA genes showed that the strains belong to the genus Kiloniella and have high similarity with the species Kiloniella spongiae (99.44–99.86%) and Kiloniella litopenaei (99.0–99.5%). Strains M56.1T (=CECT 9195, =LMG 29925), M146.1 (=CECT 9193, =LMG 29926) and SBRF 1.10 (=CECT 9194, =LMG 29927) were selected on the basis of genotyping by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). Phylogenetic analysis based on concatenated sequences of the genes gyrB, ftsZ, rpoD and mreB showed that the isolates form a differentiated branch within the genus Kiloniella. Moreover, the average nucleotide identity (ANIm, ANIb and OrthoANI) and in silico estimated DNA–DNA reassociation values between selected Galician isolates and Kiloniella species were below the established cut-off for species deliniation. The results obtained in the genetic and phenotypical analyses indicate that the isolates represent a new species of the genus Kiloniella, for which the name Kiloniella majae sp. nov. is proposed with strain M56.1T (=CECT 9195T, =LMG 29925T) as the type strain
PB Elsevier
SN 0723-2020
SN 1618-0984
YR 2017
FD 2017-07-01
LK https://hdl.handle.net/10347/46158
UL https://hdl.handle.net/10347/46158
LA eng
NO Diego Gerpe, Noemí Buján, Ana L. Diéguez, Aide Lasa, Jesús L. Romalde, Kiloniella majae sp. nov., isolated from spider crab (Maja brachydactyla) and pullet carpet shell clam (Venerupis pullastra), Systematic and Applied Microbiology, Volume 40, Issue 5, 2017, Pages 274-279, ISSN 0723-2020, https://doi.org/10.1016/j.syapm.2017.05.002
NO This work was supported in part by grants AGL2013-42628-R and AGL2016-77539-R (AEI/FEDER UE) from the Agencia Estatal de Investigación (Spain). D.G acknowledges the Ministerio de Economía y Competitividad (Spain) for a research fellowship
DS Minerva
RD 27 may 2026