RT Journal Article
T1 Antiplaque Effect of Essential Oils and 0.2% Chlorhexidine on an In Situ Model of Oral Biofilm Growth: A Randomised Clinical Trial
A1 Quintas González, Víctor
A1 Prada López, Isabel
A1 Donos, Nikolaos
A1 Suárez Quintanilla, David
A1 Tomás Carmona, Inmaculada
AB ObjectiveTo evaluate the in situ antiplaque effect after 4 days of using of 2 commercial antimicrobialagents in short term on undisturbed plaque-like biofilm.Trial Design and ParticipantsAn observer-masked, crossover randomised clinical trial on 15 oral and systemicallyhealthy volunteers between 20–30 years who were randomly and sequentially allocated inthe same group which performed 3 interventions in different randomised sequences.InterventionThe participants wore an appliance in 3 different rinsing periods doing mouthwashes twicea day (1/0/1) with essential oils, 0.2% chlorhexidine or sterile water (negative control). At theend of each 4-day mouthwash period, samples were removed from the appliance. Posteriorly, after bacterial vital staining, samples were analysed using a Confocal Laser ScanningMicroscope.Main Outcome MeasuresBacterial vitality, thickness and covering grade by the biofilm after 4 days of applying eachof the mouthwashes.ResultsThe essential oils and the 0.2% chlorhexidine were significantly more effective than the sterile water at reducing bacterial vitality, thickness and covering grade by the biofilm. No significant differences were found between the 0.2% chlorhexidine and the essential oils atreducing the bacterial vitality (13.2% vs. 14.7%). However, the 0.2% chlorhexidine showedmore reduction than the essential oils in thickness (6.5 μm vs. 10.0 μm; p<0.05) and covering grade by the biofilm (20.0% vs. 54.3%; p<0.001). ConclusionThe essential oils and 0.2% chlorhexidine showed a high antiplaque effect. Although the0.2% chlorhexidine showed better results with regard to reducing the thickness and covering grade by the biofilm, both antiseptics showed a high and similar antibacterial activity.Clinical RelevanceDaily essential oils or 0.2% chlorhexidine mouthwashes are effective when reducing dentalplaque formation in the short term. Although 0.2% chlorhexidine continues to be the “goldstandard” in terms of antiplaque effect, essential oils could be considered areliable alternative.Trial RegistrationClinicalTrials.gov NCT02124655
PB PLOS
YR 2015
FD 2015
LK http://hdl.handle.net/10347/21809
UL http://hdl.handle.net/10347/21809
LA eng
NO Quintas V., Prada-López I., Donos N., Suárez-Quintanilla D., Tomás .I (2015). Antiplaque Effect of Essential Oils and 0.2% Chlorhexidine on an In Situ Model of Oral Biofilm Growth: A Randomised Clinical Trial. PLoS ONE 10(2): e0117177
NO This work was supported by project PI11/01383 from Carlos III Institute of Health (General Division of Evaluation and Research Promotion, Madrid, Spain), which is integrated in National Plan of Research, Development and Innovation (PN I+D+I 2008-2011). This project was cofinanced by European Regional Development Fund (ERDF 2007-2013)
DS Minerva
RD 25 abr 2026