RT Journal Article
T1 Innate Immunity Biomarkers for Early Detection of Keratoconus
A1 Malfeito, Mercedes
A1 Regueiro, Uxía 
A1 Pérez Mato, María
A1 Campos, Francisco
A1 Sobrino, Tomás
A1 Lema Gesto, María Isabel
K1 Biomarkers
K1 Innate immunity
K1 Toll-like receptor 2
K1 Toll-like receptor 4
K1 Unilateral keratoconus
AB Purpose: To compare toll-like receptors 2 (TLR2) and 4 (TLR4) expression in cells of corneal and conjunctival epithelium between unilateral KC patients and control subjects.Methods: Prospective cross-sectional case-control study, including 50 unilateral KC patients and 20 control subjects. TLR2 and TLR4 expression was measured with flow cytometry.Results: Mean expression of TLR2 and TLR4, in corneal and conjunctival cells, was significantly higher in KC than in subclinical and control groups (all p < 0.0001). TLR2 expression in corneal epithelial cells can predict with the highest sensitivity and specificity the probability of KC existence compared to a control (area under the curve 0.995, 95% CI: 0.987–1.000; p < 0.0001). Corneal TLR2 expression also has a predictive capacity to discriminate between subclinical KC and controls (area under the curve 0.989, 95% CI: 0.975–1.000; p < 0.0001).Conclusions: TLR2 and TLR4 expression in corneal and conjunctival epithelial cells may constitute predictive biomarkers for the early detection of KC.
PB Taylor and Francis
YR 2019
FD 2019
LK https://hdl.handle.net/10347/45635
UL https://hdl.handle.net/10347/45635
LA eng
NO Malfeito M, Regueiro U, Pérez-Mato M, Campos F, Sobrino T, Lema I. Innate Immunity Biomarkers for Early Detection of Keratoconus. Ocul Immunol Inflamm. 2019;27(6):942-948. doi:10.1080/09273948.2018.1511813
NO This is an Accepted Manuscript of an article published by Taylor & Francis in Ocular Immunology and Inflammation, Volume 27, 2019 - Issue 6, available at: https://doi.org/10.1080/09273948.2018.1511813
NO This work was supported by the Spanish Ministry of Economy and Competitiveness (Instituto de Salud Carlos III) [grant number PI14/00247]; Xunta de Galicia (Consellería de Economía e Industria) [grant number 10 PX113918156PR] and Consellería de Educación [grant number GRC2014/027]; and the European Union program ERFD. T. Sobrino (CPII17/00027) and F. Campos (CP14/00154) are researchers under a research contract with the Miguel Servet Program of Instituto de Salud Carlos III. Similarly, U. Regueiro was awarded a predoctoral Fellowship from the Xunta de Galicia [grant number ED481A-2015/001].
DS Minerva
RD 24 abr 2026