RT Journal Article
T1 Anatomical recovery of the spinal glutamatergic system following a complete spinal cord injury in lampreys
A1 Fernández López, Blanca
A1 Barreiro Iglesias, Antón
A1 Rodicio Rodicio, María Celina
AB Lampreys recover locomotion following a spinal cord injury (SCI). Glutamate is necessary to initiateand control locomotion and recent data suggest a crucial role for intraspinal neurons in functionalrecovery following SCI. We aimed to determine whether, in lampreys, axotomized spinal glutamatergicneurons, which lose glutamate immunoreactivity immediately after SCI, recover it later on and tostudy the long-term evolution and anatomical recovery of the spinal glutamatergic system after SCI.We used glutamate immunoreactivity to study changes in the glutamatergic system, tract-tracing tolabel axotomized neurons and TUNEL labelling to study cell death. Transections of the cord were madeat the level of the fifth gill. TUNEL experiments indicated that cell death is a minor contributor to theinitial loss of glutamate immunoreactivity. At least some of the axotomized neurons lose glutamateimmunoreactivity, survive and recover glutamate immunoreactivity 1 week post-lesion (wpl). Weobserved a progressive increase in the number of glutamatergic neurons/processes until an almostcomplete anatomical recovery at 10 wpl. Among all the glutamatergic populations, the population ofcerebrospinal fluid-contacting cells is the only one that never recovers. Our results indicate that fullrecovery of the glutamatergic system is not necessary for the restoration of function in lampreys.
PB Nature Publishing Group
YR 2016
FD 2016
LK http://hdl.handle.net/10347/22452
UL http://hdl.handle.net/10347/22452
LA eng
NO Fernández-López, B., Barreiro-Iglesias, A. & Rodicio, M. Anatomical recovery of the spinal glutamatergic system following a complete spinal cord injury in lampreys. Sci Rep 6, 37786 (2016). https://doi.org/10.1038/srep37786
NO Grant sponsors: Spanish Ministry of Science and Innovation; Grant number: BFU2010–17174, Spanish Ministry of Economy and Competitiveness; Grant number: BFU2014-56300-P and Xunta de Galicia; Grant number: GPC2014/030. ABI was supported by an I2C postdoctoral grant from the Xunta de Galicia (Grant number: 2016-PG008)
DS Minerva
RD 28 abr 2026