RT Journal Article
T1 Preclinical PET Study of Intravitreal Injections
A1 Fernández Ferreiro, Anxo
A1 Luaces Rodríguez, Andrea
A1 Aguiar Fernández, Pablo
A1 Pardo Montero, Juan
A1 González Barcia, Miguel
A1 García Varela, Lara
A1 Herranz, Michel
A1 Silva Rodríguez, Jesús
A1 Gil Martínez, María
A1 Bermúdez Vázquez, María Elvira
A1 Vieites Prado, Alba
A1 Blanco Méndez, José
A1 Lamas Díaz, María Jesús
A1 Gómez-Ulla de Irazazábal, Francisco Javier
A1 Ruibal Morell, Álvaro
A1 Otero Espinar, Francisco Javier
A1 González García, Francisco
K1 Intravitreal injection
K1 Radiolabeled molecules
K1 Vitreous clearance
K1 Intravitreal pharmacokinetics
K1 PET
AB PURPOSE. This work aimed at describing the time course of vitreous clearance through the useof positron emission tomography (PET) as a noninvasive tool for pharmacokinetic studies ofintravitreal injection.METHODS. The pharmacokinetic profile of intravitreal injections of molecules labeled with 18Fluorine (18F) was evaluated in adult Sprague Dawley rats by using a dedicated small-animalPET/computed tomography scanner. Different conditions were studied: three moleculesradiolabeled with 18F (18F-FDG, 18F-NaF, and 18F-Choline), three volumes of intravitrealinjections (7, 4, and 2 lL), and absence or presence of eye inflammation (uveitis).RESULTS. Our results showed that there are significant pharmacokinetic differences among theradiolabeled molecules studied but not among the injected volumes. The presence or absenceof uveitis was an important factor in vitreous clearance, since the elimination of the drug wasclearly increased when this condition is present.CONCLUSIONS. Intravitreal pharmacokinetic studies based on the use of dedicated PET imagingcan be of potential interest as noninvasive tools in ophthalmic drug development in smallanimals
PB The Association for Research in Vision and Ophthalmology
SN 0146-0404
YR 2017
FD 2017
LK http://hdl.handle.net/10347/22920
UL http://hdl.handle.net/10347/22920
LA eng
NO Anxo Fernández-Ferreiro, Andrea Luaces-Rodríguez, Pablo Aguiar, Juan Pardo-Montero, Miguel González-Barcia, Lara García-Varela, Michel Herranz, Jesús Silva-Rodríguez, María Gil-Martínez, María A. Bermúdez, Alba Vieites-Prado, José Blanco-Méndez, María Jesús Lamas, Francisco Gómez-Ulla, Álvaro Ruibal, Francisco Javier Otero-Espinar, Francisco González; Preclinical PET Study of Intravitreal Injections. Invest. Ophthalmol. Vis. Sci. 2017;58(7):2843-2851.
NO Supported by Instituto de Salud Carlos III Rio Hortega ResearchGrant CM15/00188 (AF-F) and Miguel Servet Research Grant CP12/03162 (JP-M), MICINN Ramon y Cajal RYC-2015-17430 (PA), andpartially supported by the government research funding agenciesof Xunta de Galicia, Spain (ED431C-2016-025, ED341D-R2016-032), and by ISCIII (RETICS Oftared, RD16/0008/0003 and RD12/0034/0017) cofunded by FEDER
DS Minerva
RD 24 abr 2026