RT Journal Article
T1 DNA-binding miniproteins based on zinc fingers. Assessment of the interaction using nanopores
A1 Rodríguez Villar, Jéssica
A1 Learte Aymamí, Soraya
A1 Mosquera Mosquera, Jesús
A1 Celaya, Garbiñe
A1 Rodríguez Larrea, David
A1 Vázquez Sentís, Marco Eugenio
A1 Mascareñas Cid, José Luis
AB Obtaining artificial proteins that mimic the DNA binding properties of natural transcription factors could open new ways of manipulating gene expression at will. In this context it is particularly interesting to develop simple synthetic systems. Inspired by the modularity of natural transcription factors, we have designed synthetic miniproteins that combine the zinc finger module of the transcription factor GAGA and AT-hook peptide domains. These constructs are capable of binding to composite DNA sequences of up to 14 base pairs with high affinity and good selectivity. In particular, we have synthesized three different chimeras and characterized their DNA binding properties by electrophoresis and fluorescence anisotropy. We have also used, for the first time in the study of peptide-based DNA binders, nanopore force spectroscopy to obtain further data on the DNA interaction.
PB Royal Society of Chemistry
SN 2041-6520
YR 2018
FD 2018
LK http://hdl.handle.net/10347/22612
UL http://hdl.handle.net/10347/22612
LA eng
NO Rodríguez, J., Learte-Aymamí, S., Mosquera, J., Celaya, G., Rodríguez-Larrea, D., Vázquez, M., & Mascareñas, J. (2018). DNA-binding miniproteins based on zinc fingers. Assessment of the interaction using nanopores. Chemical Science, 9(17), 4118-4123. https://doi.org/10.1039/c7sc05441f
NO This work received support from the MINECO (SAF2013-41943-R, BFU2016-81754-ERC, CTQ2015-70698-R, SAF2014-58398-R,and SAF2016-76689-R), the Xunta de Galicia (2015-CP082,ED431C 2017/19 and Centro singular de investigación de Galicia accreditation 2016–2019, ED431G/09), the Fundación AECC(IDEAS197VAZQ), the European Union (European RegionalDevelopment Fund – ERDF), and the European ResearchCouncil (Advanced Grant No. 340055). Support of the orfeocinqa network is also acknowledged. J. R. thanks the Xunta deGalicia for a PhD fellowship; D. R.-L. is a recipient of a Ramón yCajal Fellowship (RYC-2013-12799)
DS Minerva
RD 29 abr 2026