RT Journal Article
T1 Combined sterilization and fabrication of drug-loaded scaffolds using supercritical CO2 technology
A1 Santos Rosales, Víctor
A1 Magariños Ferro, Beatriz
A1 Álvarez Lorenzo, Carmen
A1 García González, Carlos A.
K1 Sterilization
K1 Spores
K1 Supercritical CO2
K1 Bone scaffold
K1 Technology transfer
AB The access of biodegradable scaffolds to the clinical arena is constrained by the absence of a suitable sterilization technique for the processing of advanced polymeric materials. Sterilization with supercritical CO2 (scCO2) may circumvent some technological limitations (e.g., low temperature, no chemical residues on the material), although scCO2 can plasticize the polymer depending on the processing conditions used. In this latter case, the integration of the manufacturing and sterilization processes is of particular interest to obtain sterile and customized scaffolds in a single step. In this work, scCO2 was exploited as a concomitantly foaming and sterilizing agent for the first time, developing a one-step process for the production of vancomycin-loaded poly(ε-caprolactone) (PCL) bone scaffolds. The effect of the CO2 contact time on the sterility levels of the procedure was investigated, and the sterilization efficiency was evaluated against dry spores (Bacillus stearothermophilus, Bacillus pumilus and Bacillus atrophaeus). Vancomycin-loaded PCL scaffolds had relevant sustained release profiles for the prophylaxis of infections at the grafted area, even those caused by methicillin-resistant Staphylococcus aureus (MRSA). The biological performance of the scaffolds was evaluated in vitro regarding human mesenchymal stem cells (hMSCs) attachment and growth. Finally, the biocompatibility and angiogenic response of the manufactured sterile scaffolds was assessed in ovo through chick chorioallantoic membrane (CAM) assays
PB Elsevier
YR 2022
FD 2022
LK http://hdl.handle.net/10347/27706
UL http://hdl.handle.net/10347/27706
LA eng
NO International Journal of Pharmaceutics 612 (2022) 121362. https://doi.org/10.1016/j.ijpharm.2021.121362
NO This research was funded by Xunta de Galicia [ED431C 2020/17], MICINN [PID2020-120010RB-I00], Consellería de Sanidade, Servizo Galego de Saúde, Axencia de Coñecemento en Saúde (ACIS, CT850A-G), Agencia Estatal de Investigación [AEI] and FEDER funds. V. Santos-Rosales acknowledges to Xunta de Galicia (Consellería de Cultura, Educación e Ordenación Universitaria) for a predoctoral research fellowship [ED481A-2018/014]
DS Minerva
RD 24 abr 2026