RT Journal Article T1 Optimization of liquid–liquid extraction of biosurfactants from corn steep liquor A1 Vecino, Xanel A1 Barbosa Pereira, Letricia A1 Devesa-Rey, Rosa A1 Cruz, José Manuel A1 Moldes, Ana B. K1 Corn steep liquor K1 Industrial stream K1 Liquid– 36 liquid process K1 Biosurfactant K1 Nutritional medium AB In this work, the optimization of the operational conditions for the chloroform-based extraction of surface-active compounds from corn steep liquor (CSL) was carried out and the nutritional properties of the remnant aqueous phase (CSL-less biosurfactant) was evaluated as microbial fermentation medium. The optimal conditions to obtain biosurfactants from CSL were as follows: chloroform/CSL ratio 2 (v/v), 56 °C at extraction times >30 min. At the optima conditions, 100 % of biosurfactant extract can be obtained from CSL, obtaining 12.0 ± 0.5 g of biosurfactant extract/Kg of CSL. The critical micelle concentration (CMC) of the biosurfactant extract was 399.4 mg L−1. This value is similar to the CMC of cetrimonium bromide (CTAB), a cationic surfactant used in the formulation of nanoparticles. The extraction of biosurfactant can be also carried out at room temperature although in this case, the extraction yield decreased about 15 %. The extraction of surface-active compounds from agroindustrial streams can suppose important advances for the bio-based surfactants industry. Biosurfactants obtained in this work are not only more eco-friendly than chemical detergents but also can be cost competitive with its chemical counterparts. Furthermore, after the extraction of surface-active compounds, CSL-less biosurfactant was found to be suitable as nutritional supplement for lactic acid bacteria, maintaining its nutritional properties in comparison with regular CSL PB Springer SN 1615-7591 YR 2015 FD 2015 LK https://hdl.handle.net/10347/39418 UL https://hdl.handle.net/10347/39418 LA eng NO Vecino, X., Barbosa-Pereira, L., Devesa-Rey, R., Cruz, J. M., & Moldes, A. B. (2015). Optimization of liquid–liquid extraction of biosurfactants from corn steep liquor. Bioprocess and Biosystems Engineering, 38(9), 1629–1637. https://doi.org/10.1007/s00449-015-1404-9 DS Minerva RD 24 abr 2026