RT Journal Article
T1 Using zebrafish embryo bioassays combined with high-resolution mass spectrometry screening to assess ecotoxicological water bodies quality status: A case study in Panama rivers
A1 Wilson, Estibali Wilkie
A1 Castro Bustelo, Verónica
A1 Chaves, Raquel
A1 Espinosa, Miguel
A1 Rodil Rodríguez, María del Rosario
A1 Quintana Álvarez, José Benito
A1 Vieira, María Natividade
A1 Santos, Miguel M.
K1 Emerging contaminants
K1 Quadrupole-time-of flight mass spectrometry (QTOF)
K1 Screening
K1 Risk assessment
K1 Toxicity testing
K1 Danio rerio
AB Several studies show that many water bodies in developing countries are increasingly affected by anthropogenic pressure, such as agricultural activities, domestic and industrial wastewater. However, data is scarce in several of such countries, including Panama. Thus, in this work, the ecotoxicological status of selected rivers in Panama with distinct input sources were evaluated using the zebrafish (Danio rerio) embryo bioassays combined with a liquid chromatography-high resolution mass spectrometry screening of contaminants of emerging concern (CECs), using a library of over 3200 chemicals. A total of 68 CECs, including pharmaceuticals and metabolites, pesticides and several industrial chemicals, could be tentatively identified. Additionally, the zebrafish embryo bioassays showed a significant increase (p < 0.05) in embryo mortality/abnormalities when incubated with water samples from two rivers, Matasnillo and Curundú (47.5% and 32%, respectively). Importantly, a positive correlation between ecotoxicological endpoints and some of the detected CECs was observed. The findings demonstrate that both rivers are under strong anthropogenic pressure, and therefore, management actions are urgently needed to decrease their level of contamination. Overall, this study further supports the use of the zebrafish embryo bioassay as a fast, high throughput approach for screening the toxicity of water samples, and highlights the advantages of combining ecotoxicological assays with high-resolution mass spectrometry to an expedite assessment of the ecotoxicological status of water bodies.
PB Elsevier
SN 0045-6535
YR 2021
FD 2021
LK http://hdl.handle.net/10347/26999
UL http://hdl.handle.net/10347/26999
LA eng
NO E.W. Wilson et al. Chemosphere, 2021, 272, 129823
NO This work was supported by the Portuguese Foundation for Science and Technology (FCT) (PTDC|CTA-AMB|31554|2017; UIDB/04423/2020; UIDP/04423/2020), the Spanish Agencia Estatal de Investigación (ref. CTM2017-84763-C3-2-R), the Galician Council of Culture, Education and Universities (ref. ED431C2017/36 and Verónica Castro predoctoral contract, ref. ED481A-2017/156), co-funded by the European Regional Development Fund (ERDF/FEDER).
DS Minerva
RD 22 abr 2026